Nanoscale polyelectrolyte complexes encapsulating mRNA and long-chained siRNA for combinatorial cancer gene therapy

Publication date: 25 August 2018Source: Journal of Industrial and Engineering Chemistry, Volume 64Author(s): Myung Goo Kim, Sung Duk Jo, Ji Hoon Jeong, Sun Hwa KimAbstractTo precisely regulate target genes that are abnormally expressed in cancers, we suggest an RNA-mediated multigene targeting system that co-encapsulates siRNA against vascular endothelial growth factor (VEGF) and mRNA encoding phosphatase and tensin homolog (PTEN). Polymerized long-chain siRNAs (L-siRNAs) formed stable and condensed nanocomplexes with mRNAs using thiolated glycol chitosans (tGCs) as gene carriers. The mRNA/L-siRNA/tGC nanocomplexes (MSNs) exhibited efficient intracellular delivery and superior anti-tumor efficiency with simultaneous up and down-regulation of PTEN and VEGF, respectively. The MSN system can be considered as a new platform for cancer gene therapy requiring accurate control of multiple gene expressions.Graphical abstract
Source: Journal of Industrial and Engineering Chemistry - Category: Chemistry Source Type: research

Related Links:

m Self-replicating single-stranded RNA viruses such as alphaviruses, flaviviruses, measles viruses, and rhabdoviruses provide efficient delivery and high-level expression of therapeutic genes due to their high capacity of RNA replication. This has contributed to novel approaches for therapeutic applications including vaccine development and gene therapy-based immunotherapy. Numerous studies in animal tumor models have demonstrated that self-replicating RNA viral vectors can generate antibody responses against infectious agents and tumor cells. Moreover, protection against challenges with pathogenic Ebola virus was obta...
Source: Molecules - Category: Chemistry Authors: Tags: Review Source Type: research
Instead of mind-boggling inventions, 2018 was the year when national governments, as well as healthcare regulators, started to embrace digital health technologies at scale. The year when Google, Amazon, Apple or Microsoft competed head-to-head for the biggest chunks on the healthcare market, and when the buzzword of the year award went to the blockchain. Here’s our guide to the top digital health stories from last year. 2018: Under the spell of cosmos and microcosmos Every year, The Medical Futurist team sits down and collects the top stories of the past 12 months in healthcare. We put the novelties under the microsc...
Source: The Medical Futurist - Category: Information Technology Authors: Tags: Business Future of Medicine Medical Professionals Patients Policy Makers Researchers Top Lists 2018 AI artificial intelligence artificial pancreas blockchain chatbot CRISPR deep learning diabetes digital health digital he Source Type: blogs
Conclusion: Our findings demonstrate that this novel nanoplatform for targeted image-guided treatment of tumor and tactfully integrated chemotherapy, photothermal therapy (PTT) and gene therapy might provide an insight for cancer theranostics.
Source: Theranostics - Category: Molecular Biology Authors: Tags: Research Paper Source Type: research
Abstract PURPOSE: In Japan, gene therapy and cellular therapy are categorized as regenerative medicine products based on the Pharmaceuticals and Medical Devices Law that was implemented in 2014. In this new law, regenerative medicine products were newly defined, and a conditional and term-limited approval system for regenerative medicine products was instituted. In addition, the Ministry of Health, Labour and Welfare instituted the SAKIGAKE (meaning pioneer or forerunner in Japanese) designation system in 2015. This designation is similar to the breakthrough therapy designation in the United States. These new regu...
Source: Clinical Therapeutics - Category: Drugs & Pharmacology Authors: Tags: Clin Ther Source Type: research
Upregulating microRNA-498 inhibits gastric cancer proliferation invasion and chemoresistance through inverse interaction of Bmi1, Published online: 05 December 2018; doi:10.1038/s41417-018-0065-7Upregulating microRNA-498 inhibits gastric cancer proliferation invasion and chemoresistance through inverse interaction of Bmi1
Source: Cancer Gene Therapy - Category: Cancer & Oncology Authors: Source Type: research
BET inhibitor I-BET151 sensitizes GBM cells to temozolomide via PUMA induction, Published online: 05 December 2018; doi:10.1038/s41417-018-0068-4BET inhibitor I-BET151 sensitizes GBM cells to temozolomide via PUMA induction
Source: Cancer Gene Therapy - Category: Cancer & Oncology Authors: Source Type: research
ACS Applied Materials&InterfacesDOI: 10.1021/acsami.8b13291
Source: ACS Applied Materials and Interfaces - Category: Materials Science Authors: Source Type: research
ConclusionDespite limited intravesical treatment options in BCG failure patients, there are several promising therapies currently being developed and several with promising early results.
Source: World Journal of Urology - Category: Urology & Nephrology Source Type: research
Abstract The number of primary immune deficiencies exceeds 350, approximately a quarter of them having neurological implications. Severe central nervous system infections may occur in an even higher proportion. Beyond listing in a table of all diseases with a neurological impact, the author gives detailed analysis of one typical disorder. Ataxia telangiectasia is caused by biallelic mutation of the ATM gene resulting in genomic instability, increased cancer risk, immune deficiency and a predominantly cerebellar neurodegeneration. The most common classic form is characterized by gait and limb ataxia, oculomotor apr...
Source: Orvosi Hetilap - Category: General Medicine Authors: Tags: Orv Hetil Source Type: research
Abstract Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third most lethal cancer worldwide. Although gene mutations associated with HCC development have been intensively studied, how epigenetic factors specifically modulate the functional properties of HCC by regulating target gene expression is unclear. Here we demonstrated the overexpression of KDM3B in liver tissue of HCC patients using public RNA-seq data. Ablation of KDM3B by CRISPR/Cas9 retarded the cell cycle and proliferation of hepatocarcinoma HepG2 cells. Approximately 30% of KDM3B knockout cells exhibited mitotic spindle multipol...
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Tags: Biochem Biophys Res Commun Source Type: research
More News: Cancer | Cancer & Oncology | Chemistry | Gene Therapy | Genetics | Nanotechnology