Thymoquinone induces apoptosis in bladder cancer cell via endoplasmic reticulum stress-dependent mitochondrial pathway.

Thymoquinone induces apoptosis in bladder cancer cell via endoplasmic reticulum stress-dependent mitochondrial pathway.
Chem Biol Interact. 2018 Jul 02;:
Authors: Zhang M, Du H, Huang Z, Zhang P, Yue Y, Wang W, Liu W, Zeng J, Ma J, Chen G, Wang X, Fan J
Abstract
BACKGROUND: Thymoquinone (TQ), the major active compound isolated from black seed oil (Nigella sativa), has been reported to exhibit anti-inflammatory and anticancer abilities. However, the exact molecular mechanism underlying the anticancer effect of TQ is still poorly understood, especially in regard to TQ's effect on endoplasmic reticulum stress-mediated apoptosis.
METHODS: The cytotoxicity of TQ on T24 and 253J bladder cancer cells was analyzed by MTT assay, colony formation assay and Annexin V-FITC/PI staining, while SV-HUC-1, the SV-40 immortalized human uroepithelial cell line, was used as a normal control. The change in mitochondrial membrane potential was assessed by JC-1 staining. mRNA expression and protein expression were detected by qPCR and western blotting. Caspase-3 activity was detected by colorimetric assay.
KEY FINDING: TQ has a significant cytotoxicity on bladder cancer cells and can inhibit their proliferation and induce apoptosis. The protein changes of Bcl-2, Bax, cytochrome c and endoplasmic reticulum stress-related proteins (GRP78, CHOP, and caspase-12) revealed that the anticancer effect of TQ was associated with mitochondrial dysfunc...

Source: Chemico-Biological Interactions - July 2, 2018 Category: Molecular Biology Authors: Zhang M, Du H, Huang Z, Zhang P, Yue Y, Wang W, Liu W, Zeng J, Ma J, Chen G, Wang X, Fan J Tags: Chem Biol Interact Source Type: research