Protective effect of berberine against cardiac ischemia/reperfusion injury by inhibiting apoptosis through the activation of Smad7
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Weidong Yao, Xin Wang, Kun XiaoAbstractBerberine (BBR) is an isoquinnoline derivative alkaloid extracted from Rhizoma Coptidis that has the potential to protect myocardial tissues from ischemia/reperfusion (I/R) injuries. We attempted to evaluate the effect of BBR on the proliferation and apoptosis of a hypoxia/reoxygenation (H/R) cell model and to reveal the mechanism driving the improving function of BBR myocardial tissues. The H/R cell model was established using H9c2 rat cardiac myoblasts. The cell viability, apoptotic rates, and cell cycle distribution were measured with CCK-8 assay and flow cytometry. The expression of Smad7 and caspase-3 were determined both at mRNA and protein levels. In addition, expression of Smad7 was knocked down with specific siRNA and the effect of the interference was assessed. The proliferation ability of H/R cells was enhanced after the administration of BBR, and the apoptosis and cell cycle arrest due to H/R injury were also alleviated by BBR treatment. Moreover, the treatment of BBR on H/R injury functioned through the Smad7-activation-induced attenuating of apoptosis by activating Smad7 pathway which resulted suppression of caspase 3 expression and activity. The knockdown of Smad7 confirmed our conclusion about the key role of Smad7 in the function of BBR administration. However, our results as well as some previous studies also demonstrated that the eff...
Publication date: Available online 24 April 2019Source: Journal of Critical CareAuthor(s): Philippe Montravers, Ignacio Martin-Loeches
Publication date: Available online 25 April 2019Source: European Annals of Otorhinolaryngology, Head and Neck DiseasesAuthor(s): G. Dumas, H. Tan, L. Dumas, P. Perrin, A. Lion, S. SchmerberAbstractObjectiveTo establish optimum stimulus frequency and location of bone conducted vibration provoking a skull vibration induced nystagmus (SVIN) in superior semi-circular canal dehiscences.MethodsSVIN 3D components in 40 patients with semi-circular canal dehiscence (27 unilateral and 13 bilateral) were compared with a group of 18 patients with severe unilateral vestibular loss and a control group of 11 volunteers.ResultsIn unilater...
Conclusion: The high IGF-II/IGFBP-3 molar ratio was not the cause of the sustained high growth velocity, due to the low affinity of IGF-II for IGF type 1 receptor. The key finding was theHESX1 mutation, as similar cases have been described before, suggesting a common mechanism for growth without GH. However, the variable penetrance of this variant in heterozygous relatives suggests that modifier genes or mechanisms involving combinations with mutations of other genes involved in intracellular signaling pathways might be responsible.Horm Res Paediatr
Publication date: Available online 24 April 2019Source: The Lancet Diabetes &EndocrinologyAuthor(s): Stuart W Flint
Publication date: Available online 24 April 2019Source: The Lancet Diabetes &EndocrinologyAuthor(s): Peter H Bennett
Publication date: Available online 13 April 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Matti Poutanen, Trevor M. Penning
ConclusionSGLT2 inhibitors have relatively safe profiles and can efficiently decrease HbA1c as well as fasting and postprandial glucose levels. Furthermore, SGLT2 inhibitors administrations are not associated with significant hypoglycemic episodes or weight gain. Thus, combination of SGLT2 inhibitors and other less harmful anti-diabetic medicines could be considered if there is no any contraindication.
ConclusionDiabetes was correlated significantly with dyslipidemia and atherogenic risk in parallel with an increase in IFN-γ production and hematological inflammatory biomarkers; leukocytes, neutrophil/lymphocyte and platelet/lymphocyte ratios, MPV and PDW values. The amelioration in inflammatory biomarkers was associated with improvement in glycemic control.
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