Protective effect of berberine against cardiac ischemia/reperfusion injury by inhibiting apoptosis through the activation of Smad7

Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Weidong Yao, Xin Wang, Kun XiaoAbstractBerberine (BBR) is an isoquinnoline derivative alkaloid extracted from Rhizoma Coptidis that has the potential to protect myocardial tissues from ischemia/reperfusion (I/R) injuries. We attempted to evaluate the effect of BBR on the proliferation and apoptosis of a hypoxia/reoxygenation (H/R) cell model and to reveal the mechanism driving the improving function of BBR myocardial tissues. The H/R cell model was established using H9c2 rat cardiac myoblasts. The cell viability, apoptotic rates, and cell cycle distribution were measured with CCK-8 assay and flow cytometry. The expression of Smad7 and caspase-3 were determined both at mRNA and protein levels. In addition, expression of Smad7 was knocked down with specific siRNA and the effect of the interference was assessed. The proliferation ability of H/R cells was enhanced after the administration of BBR, and the apoptosis and cell cycle arrest due to H/R injury were also alleviated by BBR treatment. Moreover, the treatment of BBR on H/R injury functioned through the Smad7-activation-induced attenuating of apoptosis by activating Smad7 pathway which resulted suppression of caspase 3 expression and activity. The knockdown of Smad7 confirmed our conclusion about the key role of Smad7 in the function of BBR administration. However, our results as well as some previous studies also demonstrated that the eff...
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research

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Source: Fungal Biology - Category: Biology Source Type: research
Publication date: Available online 16 November 2018Source: Biological ControlAuthor(s): Lindsey R. Milbrath, Jeromy Biazzo, Antonio DiTommaso, Scott H. MorrisAbstractThe moth Abrostola asclepiadis (Lepidoptera: Noctuidae) is being investigated for the biological control of pale and black swallow-wort (Vincetoxicum rossicum and V. nigrum, respectively; Apocynaceae), European vines introduced into North America. Limited information existed on its potential impact on swallow-worts, and the combination of defoliation damage by A. asclepiadis and other stresses to the plants had not been studied. We conducted greenhouse studies...
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Source: Nitric Oxide - Category: Chemistry Source Type: research
Publication date: Available online 17 November 2018Source: Journal of Environmental Chemical EngineeringAuthor(s): A. Rosset, Plantard G., Djessas K.AbstractSolar applications in the treatment of waste water fields were limited by the fact that the photocatalytic efficiency of photoreactors, and specifically the photocatalyst used, was less than one percent. Here we report a study investigating the catalysts based on Zn1-xMxO compounds for different [M]/[Zn] atomic ratios. M elements: Na, K, Li, Fe, V, Co, Mg, Mn, Ca, In, Al, P, Ce, were selected. All these catalysts were synthesized by sol-gel process. The morphological, ...
Source: Journal of Environmental Chemical Engineering - Category: Chemistry Source Type: research
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Source: Journal of Environmental Chemical Engineering - Category: Chemistry Source Type: research
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Source: Chinese Journal of Catalysis - Category: Chemistry Source Type: research
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Source: Chinese Journal of Catalysis - Category: Chemistry Source Type: research
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Source: Chinese Journal of Catalysis - Category: Chemistry Source Type: research
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