Development of a TaqMan-based real-time PCR assay for the rapid and specific detection of pigeon torque teno virus

Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Chunhe Wan, Cuiteng Chen, Longfei Cheng, Guanghua Fu, Shaohua Shi, Qiuling Fu, Rongchang Liu, Hongmei Chen, Yu HuangAbstractPigeon torque teno virus (PTTV), a recently discovered circular DNA virus. Here, we developed a TaqMan-based real-time PCR for rapid and specific detection of PTTV infections with sensitivity up to 49.3 copies/μl. Positive signals can be observed by the assay in pigeon embryonated eggs, which indicted that PTTV can be transmitted vertically. Our findings play important implications for a better understanding the transmission of torque teno virus in pigeons.

Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research