Carbon monoxide releasing molecule-2 attenuates Pseudomonas aeruginosa-induced ROS-dependent ICAM-1 expression in human pulmonary alveolar epithelial cells

Publication date: September 2018Source: Redox Biology, Volume 18Author(s): Chiang-Wen Lee, Cheng-Hsun Wu, Yao-Chang Chiang, Yuh-Lien Chen, Kuo-Ting Chang, Chu-Chun Chuang, I-Ta LeeAbstractPseudomonas aeruginosa (P. aeruginosa) infection in the lung is common in patients with cystic fibrosis (CF). Intercellular adhesion molecule-1 (ICAM-1) is known to play a key role in lung inflammation. Acute inflammation and its timely resolution are important to ensure bacterial clearance and limit tissue damage. Carbon monoxide (CO) has been shown to exert anti-inflammatory effects in various tissues and organ systems. Here, we explored the protective effects and mechanisms of carbon monoxide releasing molecule-2 (CORM-2) on P. aeruginosa-induced inflammatory responses in human pulmonary alveolar epithelial cells (HPAEpiCs). We showed that P. aeruginosa induced prostaglandin E2 (PGE2)/interleukin-6 (IL-6)/ICAM-1 expression and monocyte adherence to HPAEpiCs. Moreover, P. aeruginosa-induced inflammatory responses were inhibited by transfection with siRNA of Toll-like receptor 4 (TLR4), PKCα, p47phox, JNK2, p42, p50, or p65. P. aeruginosa also induced PKCα, JNK, ERK1/2, and NF-κB activation. We further demonstrated that P. aeruginosa increased intracellular ROS generation via NADPH oxidase activation. On the other hand, P. aeruginosa-induced inflammation was inhibited by pretreatment with CORM-2. Preincubation with CORM-2 had no effects on TLR4 mRNA levels in response to P...
Source: Redox Biology - Category: Biology Source Type: research

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Conclusions: The present review discusses a novel mechanism for pulmonary edema fluid reabsorption. SPMs might provide new opportunities to design "reabsorption-targeted" therapies with high degrees of precision in controlling ALI/ARDS. PMID: 29664060 [PubMed - in process]
Source: Chinese Medical Journal - Category: General Medicine Authors: Tags: Chin Med J (Engl) Source Type: research
Conclusions: The intra-session variability of DL, NO, DL, CO, DM, CO and Vc improves with breath-hold maneuver training in otherwise naìˆve CF patients, indicating a considerable learning effect. However, acceptability criteria were fulfilled at the first session. Inter-session reproducibility data are comparable to those previously reported in healthy subjects.
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Respiratory Function Technologists/Scientists Source Type: research
Publication date: Available online 3 August 2017 Source:Respiratory Physiology & Neurobiology Author(s): Thomas Radtke, Christian Benden, Marion Maggi-Beba, Susi Kriemler, Ivo van der Lee, Holger Dressel We evaluated the intra-session and inter-session variability of the diffusing capacity of nitric oxide (DLNO), carbon monoxide (DLCO), membrane diffusing capacity for carbon monoxide (DMCO) and pulmonary capillary blood volume (Vc) in patients with cystic fibrosis (CF). Patients performed single-breath diffusing capacity measurements during all of 3 consecutive study visits. Precision of gas diffusing parameters was q...
Source: Respiratory Physiology and Neurobiology - Category: Respiratory Medicine Source Type: research
DISCUSSION SESSION 1: Food allergy (PD01 –PD05)PD01 Allergen-specific humoral and cellular responses in children who fail egg oral immunotherapy due to allergic reactionsMarta Vazquez-Ortiz, Mariona Pascal, Ana Maria Plaza, Manel JuanPD02 FoxP3 epigenetic features in children with cow milk allergyLorella Paparo, Rita Nocerino, Rosita Aitoro, Ilaria Langella, Antonio Amoroso, Alessia Amoroso, Carmen Di Scala, Roberto Berni CananiPD04 Combined milk and egg allergy in early childhood: let them eat cake?Santanu Maity, Giuseppina Rotiroti, Minal GandhiPD05 Introduction of complementary foods in relation to allergy and gut...
Source: Clinical and Translational Allergy - Category: Allergy & Immunology Source Type: research
We present a case of inaccurate carboxy-hemoglobin measurement by pulse CO-oximetry due to digital clubbing. An 18-year-old man with a history of cystic fibrosis presented after a suicide attempt by inhalation of exhaust. At the initial emergency department evaluation, his blood carboxyhemoglobin was 33%. He was intubated, placed on 100% oxygen and transferred to our facility. Upon arrival, we placed three different pulse CO-oximeters on different fingers and toes. Carboxyhemoglobin levels measured by these meters ranged from 9%-11%. A venous blood gas drawn on arrival showed a carboxyhemoglobin level of 2.3% after four ho...
Source: Undersea and Hyperbaric Medicine - Category: Sports Medicine Authors: Tags: Undersea Hyperb Med Source Type: research
Background: Few studies have assessed the role of lung diffusing capacity (diffusing capacity of the lung for carbon monoxide (DLCO)) in non-CF bronchiectasis (BR). We sought to examine the relationship between DLCO and other variables in an Irish population of BR patients and determine its potential prognostic significance.Methods: DLCO data was available in 195/212 (92%) consecutive BR patients attending our institution over a 2-year period. Univariate analyses was performed using Pearson's correlation. Stepwise logistic regression analyses was subsequently performed to determine if DLCO was an independent predictor of d...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: 10.1 Respiratory Infections Source Type: research
Background: International guidelines recommend the inclusion of patients with bronchiectasis in pulmonary rehabilitation (PR) to improve exercise capacity and health-related quality of life (HRQoL). At present, the effect of PR in these patients has been poorly investigated. Objective: The aim of our retrospective analysis was to evaluate the effects and predictors of success for a PR program in patients with bronchiectasis not related to cystic fibrosis (non-CF bronchiectasis). Methods: One hundred and thirty-five non-CF bronchiectasis inpatients, allocated to a 3-week PR program, were retrospectively evaluated. Exercise ...
Source: Respiration - Category: Respiratory Medicine Source Type: research
Conclusion Adults with CF have reduced quality of life, which in part is associated with the severity of their lung function.
Source: Revista Portuguesa de Pneumologia - Category: Respiratory Medicine Source Type: research
Publication date: Available online 20 January 2015 Source:Redox Biology Author(s): Maher Y. Abdalla , Iman M. Ahmad , Barbara Switzer , Bradley E. Britigan Mycobacterium abscessus (M.abs) is a rapidly growing mycobacterial species that infects macrophages, and is an important pathogen in patients with cystic fibrosis. We studied the early stages of M.abs infection of macrophages, with emphasis on the role of heme-oxygenase-1 (HO-1) in this infection. THP-1 cells were activated using TPA into macrophage-like cells and infected with M.abs for different time points. M.abs infection robustly induced HO-1 expression in the TH...
Source: Redox Biology - Category: Biology Source Type: research
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