Immobilization of trypsin onto Fe3O4@SiO2 –NH2 and study of its activity and stability

Publication date: 1 October 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 170Author(s): Esmaeil Aslani, Abdolreza Abri, Mohammad PazhangAbstractThe preparation of biocatalysts based on immobilized trypsin is of great importance for proteomic research, industrial applications and organic synthesis. Here in, we have developed a facile method to immobilize trypsin on magnetic nanoparticles. Fe3O4 nanoparticles were synthesized by co-precipitating Fe2+and Fe3+in an ammonia solution and then coated by silicon dioxides were developed by sol–gel method. The silica-coated Fe3O4 nanoparticles were further modified with 3-aminopropyltriethoxysilane, resulting in attaching of primary amine groups on the surface of the particles. Trypsin from porcine pancrease was then immobilized on the magnetic core-shell particles by using glutaraldehyde as a cross-linker. The synthesis steps and characterizations of immobilized trypsin were examined by FT-IR, XRD, TGA, EDX and SEM. The results showed that the enzyme immobilization increased the enzyme activity in different pHs and temperatures, without any changes in the optimum pH and temperature for enzyme activity. The Kinetic results showed that the enzyme immobilization decreased and increased Vmax and Km values, respectively. The stability results showed that the enzyme immobilization improved trypsin thermostability in the absence and presence of 10% (v/v) of the used solvents (DMF, THF, DMSO, ACN and 1, 4-Dioxane). The reusabil...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research