Paper based analytical device modified with nanoporous material for the fluorescent sensing of gliadin content in different food samples

Publication date: November 2018Source: Microchemical Journal, Volume 142Author(s): Evelyn Marín-Barroso, Cristian M. Moreira, Germán A. Messina, Franco A. Bertolino, Mara Alderete, Galo J.A.A. Soler-Illia, Julio Raba, Sirley V. PereiraAbstractA novel fluorescent paper based immunosensor for the quantification of gliadin content in different food samples was constructed. The device consists of a paper platform modified with amino functionalized mesoporous material. The nanoporous structure and the aminofunctionality increased the anti-gliadin antibodies immobilization capacity of the sensing surface, conferring high sensitivity to the system. The detection limit reached by the described system allowed us to address the control of gluten free foods, which is extremely important to maintain the food safe consumption by patients with celiac disease, wheat allergy measured by immunoglobulin E, non-celiac gluten intolerance and Dermatitis herpetiformis or Duhring disease. The gliadin determination was performed by applying a noncompetitive immunoassay format, where gliadin present in food samples was recognized by anti-gliadin antibodies immobilized on the mesoporous material and quantified by the addition of anti-gliadin antibody labelled with peroxidase, its substrate: hydrogen peroxide and a mediator: 10-acetyl-3,7-dihydrofenoxacin. This mediator by the action of the enzyme generates resorufin, which was excited by a light emitting diode at 550 nm and emitted a signal at 580...
Source: Microchemical Journal - Category: Chemistry Source Type: research