Evaluation of siRNA and Cationic Liposomes Complexes as a Model for in vitro siRNA Delivery to Cancer Cells

This study aims to investigate the effect of different molar charge ratios (R+/-) between positive charges from microfluidics-produced cationic liposomes (CL) (egg phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) and negative charges from siRNA and on physico-chemical and morphological properties of the lipoplexes (CL/siRNA) as well as their in vitro luciferase silencing effect in HeLa cells. R+/- 3.27 was found to be the optimum point for complexation. This finding was confirmed by gel retardation and siRNA accessibility assays. According to Cryo-TEM analysis, the lipoplexes had multi-lamellarity. In vitro transfection efficiency of lipoplexes in HeLa cells was tested at three different siRNA concentrations (10, 25, and 35 nM). Significant knockdown of luciferase by siRNA lipoplexes was observed based on reduced luciferase activity of transfected HeLa cells. Our findings were comparable with the silencing effect of siRNA complexed with Lipofectamine®. No cytotoxicity of lipoplexes was detected at the tested concentrations. This study was essential for further complexation studies which will be performed using microfluidic systems to formulate next-generation lipid-based controlled release systems.Graphical Abstract
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research

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Fight Aging! provides a weekly digest of news and commentary for thousands of subscribers interested in the latest longevity science: progress towards the medical control of aging in order to prevent age-related frailty, suffering, and disease, as well as improvements in the present understanding of what works and what doesn't work when it comes to extending healthy life. Expect to see summaries of recent advances in medical research, news from the scientific community, advocacy and fundraising initiatives to help speed work on the repair and reversal of aging, links to online resources, and much more. This content is...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Publication date: Available online 15 November 2018Source: International Journal of PharmaceuticsAuthor(s): Wenxing Song, David A. Gregory, Haider Al-janabi, Munitta Muthana, Zhiqiang Cai, Xiubo ZhaoAbstractThe lack of efficient and cost-effective methods for gene delivery has significantly hindered the applications of gene therapy. In this paper, a simple one step and cost effective salting-out method has been explored to fabricate silk-PEI nanoparticles (SPPs) and magnetic-silk/PEI core-shell nanoparticles (MSPPs) for targeted delivery of c-myc antisense oligodeoxynucleotides (ODNs) into MDA-MB-231 breast cancer cells. T...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
We describe phenomena using science, which gives us a sense of understanding and structure – yet we often lack actual understanding about what we’re observing, or why our treatments work. We have scientific explanations that may appear solid at first glance, but are flimsy upon closer inspection. More commonly, I imagine, we rely on scientific explanations as heuristics to enable us to get through our days, as a scaffold upon which to organize our information. I suspect AI is viscerally uncomfortable, and challenging to apply to clinical care or drug discovery (see part 2), because of the psychological importan...
Source: The Health Care Blog - Category: Consumer Health News Authors: Tags: Artificial Intelligence Pharmaceuticals Physicians AI David Shaywitz Health Tech Source Type: blogs
Researchers here report on two new senolytic compounds identified in the existing library of approved drugs, based on screening work in cell cultures. It is worth bearing in mind that drug candidates that demonstrate good results in cell culture quite often fail to show promise when tested in animals, so it is wise to be patient as new senolytics work their way through the research and development pipeline. There will be a lot more of this sort of thing in the years ahead, as ever greater amounts of funding pour into finding new ways to selectively destroy senescent cells. Any senolytic approach that removes a significant ...
Source: Fight Aging! - Category: Research Authors: Tags: Daily News Source Type: blogs
Publication date: January–February 2019Source: Reports of Practical Oncology &Radiotherapy, Volume 24, Issue 1Author(s): Rut Cañas, Isabel Linares, Ferran Guedea, Miguel Ángel BerenguerAbstractRadiological Oncology, like the rest of medical specialties, is beginning to provide can personalized therapies. The ongoing scientific advances enable a great degree of precision in diagnoses and therapies. To fight cancer, from a radiotherapy unit, requires up-to-date equipment, professionals with different specialties working in synchrony (doctors, physicists, biologists, etc.) and a lot of research. Some o...
Source: Reports of Practical Oncology and Radiotherapy - Category: Cancer & Oncology Source Type: research
Human life expectancy is increasing on a global scale, but healthspan—the period of life free from age-associated ill health—is not improving at a comparable rate. This disconnect means that a greater proportion of the general population will spend a longer period of their life suffering from one or more debilitating age-associated diseases, such as cardiovascular disease, Alzheimer's disease, osteoporosis, sarcopenia and various cancers. Understanding the processes underlying ageing and age-related diseases is therefore a major and pressing research challenge in biomedical research.
Source: EMBO Journal - Category: Molecular Biology Authors: Tags: Ageing, Genetics, Gene Therapy & Genetic Disease Commentary Source Type: research
Functional Improvement of Chimeric Antigen Receptor Through Intrinsic Interleukin-15Rα Signaling. Curr Gene Ther. 2018 Nov 15;: Authors: Nair S, Wang JB, Tsao ST, Liu Y, Zhu W, Slayton WB, Moreb JS, Dong L, Chang LJ Abstract Recent studies of CD19-specific chimeric antigen receptor (CAR)-modified T cells (CARTs) have demonstrated unprecedented successes in treating refractory and relapsed B cell malignancies. The key to the latest CART therapy advances can be attributed to the improved co-stimulatory signals in the CAR design. Here we established several novel CARs by incorporating T cell signal...
Source: Current Gene Therapy - Category: Genetics & Stem Cells Authors: Tags: Curr Gene Ther Source Type: research
Bacteria-mediated delivery of RNAi effector molecules against viral HPV16-E7 eradicates oral squamous carcinoma cells (OSCC) via apoptosis, Published online: 15 November 2018; doi:10.1038/s41417-018-0054-xBacteria-mediated delivery of RNAi effector molecules against viral HPV16-E7 eradicates oral squamous carcinoma cells (OSCC) via apoptosis
Source: Cancer Gene Therapy - Category: Cancer & Oncology Authors: Source Type: research
Human Gene Therapy, Ahead of Print.
Source: Human Gene Therapy - Category: Genetics & Stem Cells Authors: Source Type: research
Today, I'll point out an analysis from the SENS Research Foundation that covers the approach to selective destruction of senescent cells taken by one of the newly formed biotech startups in the space, Senolytic Therapeutics. This field is hot because it is now well proven that senescent cells are the enemy. They are one of the root causes of aging, accumulating with age to degrade tissue function via the secretion of inflammatory signal molecules. Senescent cells actively maintain an aged, inflamed state of metabolism, resulting in the development of age-related disease and increased mortality. Senescent cells do se...
Source: Fight Aging! - Category: Research Authors: Tags: Medicine, Biotech, Research Source Type: blogs
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