Peptide stabilized gold and silver nanoparticles derived from the mangrove isolate Pseudoalteromonas lipolytica mediate dye decolorization

Publication date: Available online 30 June 2018Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Rasika Kulkarni, Shubham Harip, Ameeta Ravi Kumar, Deepti Deobagkar, Smita ZinjardeABSTRACTA mangrove associated halotolerant bacterium identified as Pseudoalteromonas lipolytica mediated the synthesis of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). Interaction of P. lipolytica cells with chloroauric acid (HAuCl4) resulted in the synthesis of cell associated AuNPs that could be released in an extracellular manner after sonication. For obtaining extracellular AgNPs, pre-grown cells were subjected to osmotic shock treatment by re-suspending cells in distilled water. The preparation was centrifuged and the filtered supernatant was challenged with 4 mM AgNO3. The biogenic NPs were characterized on the basis of UV-Visible spectroscopy, Field Emission Scanning Electron Microscope observations, Transmission Electron Microscope studies, X-Ray Diffraction profiles and Energy-Dispersive X-ray spectroscopy. The size of AuNPs and AgNPs ranged between 10-30 nm and 5-15 nm, respectively. The active components in the preparation after osmotic shock treatment were subjected to thin layer chromatography, Fourier Transform Infrared spectroscopy and liquid chromatography mass spectrometry (LC-MS) analysis. Five oligopeptides, Ile-Arg, His-Asp-Asp, Glu-Ile-Lys, Lys-Asp-Asn, and Arg-Arg-Gln were identified in the active band. AuNPs brought about 9...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research