GC quantitative analysis of benzyl isothiocyanate in Salvadora persica roots extract and dental care herbal products

Publication date: May 2018Source: Saudi Pharmaceutical Journal, Volume 26, Issue 4Author(s): Maged Saad Abdel-Kader, Elsadig Hassan Khamis, Ahmed Ibrahim Foudah, Mohammed Hamed AlqarniAbstractAn accurate, sensitive, precise and simple method was developed utilizing Gas Chromatography for the quantitative analysis of benzyl isothicyanate in Siwak extract and dental care herbal products claimed to contain Siwak. Rtx (30.0 m × 0.25 mm ID, 25 µm thickness) column was used and helium as carrier gas at a flow rate of 0.74 mL/min. The retention time of standard benzyl isothicyanate was 13.470 min under the described conditions. Linear regression data analysis indicated a good linear relationship between peak height measurement and concentration of benzyl isothiocyanate in the range of 10–50 µg/ml (R2 = 0.9971). The regression equation was y = 11,471x. The developed GC method was subjected to validation requirements set by the ICH for precision, accuracy, and robustness. The entitled GC analyses expected to be valuable for the determination of benzyl isothiocyanate in Siwak extracts and other formulations containing Siwak extract. The amount of benzyl isothiocyanate reflects the efficacy of the products.
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research