miR-149 regulates the proliferation and apoptosis of cervical cancer cells by targeting GIT1

In this study, miR-149 expression is decreased in CC tissues and cell lines compare with paired normal tissues and normal epithelial cell, respectively. Moreover, the restoration of miR-149 expression inhibited cell proliferation, migration, invasion and promoted cell apoptosis in vitro. We further proved that miR-149 overexpression suppresse the growth of cervical cancer cells in vivo using a mouse xenograft model. Dual luciferase assays identified the GIT1 as a novel direct target of miR-149. To identify the mechanisms, we investigated the PI3K/AKT/mTOR pathway and found that the expression of PI3K, AKT and mTOR were suppressed in cells which were transfected with miR-149 mimics.Taken together, these results indicate that miR-149 is involved in the carcinogenesis and development of cervical cancer by targeting GIT1 via PI3K/AKT/mTOR pathway. Given these, our data suggest that miR-149 functions as a tumor suppressor and may serve as a biomarker or useful therapeutic target of cervical cancer.Graphical abstract
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research