A naked mole-rat iPSC line expressing drug-inducible mouse pluripotency factors developed from embryonic fibroblasts

Publication date: Available online 20 June 2018Source: Stem Cell ResearchAuthor(s): Sang-Goo Lee, Aleksei E. Mikhalchenko, Sun Hee Yim, Vadim N. GladyshevAbstractNaked mole rats (NMRs, Heterocephalus glaber) are long-lived, cancer-resistant rodents. Here, we report the development of an induced pluripotent stem cell (iPSC) line generated from immortalized NMR embryonic fibroblasts transduced with a doxycycline-inducible mouse OSKM polycistronic vector. This iPSC line was shown to express pluripotency-associated markers, form embryoid bodies, differentiate in vitro to the derivatives of three germ layers, and exhibit normal karyotype. The ability of iPSCs to differentiate in vivo was supported by the contribution to interspecific chimera upon injection into mouse blastocysts. This NMR iPSC line may be a useful tool in cancer and aging research.
Source: Stem Cell Research - Category: Stem Cells Source Type: research