The fungal alkaloid Okaramine-B activates an L-glutamate-gated chloride channel from Ixodes scapularis, a tick vector of Lyme disease

Publication date: August 2018Source: International Journal for Parasitology: Drugs and Drug Resistance, Volume 8, Issue 2Author(s): Shogo Furutani, Makoto Ihara, Kristin Lees, Steven D. Buckingham, Frederick A. Partridge, Jonathan A. David, Rohit Patel, Scott Warchal, Ian R. Mellor, Kazuhiko Matsuda, David B. SattelleAbstractA novel L-glutamate-gated anion channel (IscaGluCl1) has been cloned from the black-legged tick, Ixodes scapularis, which transmits multiple pathogens including the agents of Lyme disease and human granulocytic anaplasmosis. When mRNA encoding IscaGluCl1 was expressed in Xenopus laevis oocytes, we detected robust 50–400 nA currents in response to 100 μM L-glutamate. Responses to L-glutamate were concentration-dependent (pEC50 3.64 ± 0.11). Ibotenate was a partial agonist on IscaGluCl1. We detected no response to 100 μM aspartate, quisqualate, kainate, AMPA or NMDA. Ivermectin at 1 μM activated IscaGluCl1, whereas picrotoxinin (pIC50 6.20 ± 0.04) and the phenylpyrazole fipronil (pIC50 6.90 ± 0.04) showed concentration-dependent block of the L-glutamate response. The indole alkaloid okaramine B, isolated from fermentation products of Penicillium simplicissimum (strain AK40) grown on okara pulp, activated IscaGluCl1 in a concentration-dependent manner (pEC50 5.43 ± 0.43) and may serve as a candidate lead compound for the development of new acaricides.Graphical abstract
Source: International Journal for Parasitology: Drugs and Drug Resistance - Category: Parasitology Source Type: research

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(CBS News) – New government research shows that tickborne diseases like Lyme disease continue to rise. The report from the Centers for Disease Control and Prevention finds that in 2017, state and local health departments reported a record number of cases of illnesses spread by ticks. Cases of Lyme disease, Powassan virus; spotted fever rickettsiosis, including Rocky Mountain spotted fever, anaplasmosis, ehrlichiosis, babesiosis and tularemia all increased last year. The CDC said there were 59,349 confirmed cases of tickborne diseases in 2017, up from 48,610 in 2016. In past years, h...
Source: WBZ-TV - Breaking News, Weather and Sports for Boston, Worcester and New Hampshire - Category: Consumer Health News Authors: Tags: Health News CBS News LYME DISEASE ticks Source Type: news
Publication date: Available online 17 October 2018Source: Ticks and Tick-borne DiseasesAuthor(s): Christine A. James, David L. Pearl, L. Robbin Lindsay, Andrew S. Peregrine, Claire M. JardineAbstractIn eastern North America, the blacklegged tick, Ixodes scapularis, is the vector for Borrelia burgdorferi sensu stricto and Anaplasma phagocytophilum, the causal agents for human and canine Lyme disease and anaplasmosis, respectively. The extensive range expansion of I. scapularis in Ontario is a growing veterinary and public health concern. However, there is limited information on the risk factors associated with I. scapularis...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
This article discusses case examples showing the wide range of symptoms and presentations that are possible with babesiosis.
Source: The Nurse Practitioner - Category: Nursing Tags: Feature: TICK-BORNE INFECTION Source Type: research
Pilot study of Ixodes ricinus ticks preference for human ABO blood groups using a simple in vitro method. Ann Agric Environ Med. 2018 Jun 20;25(2):326-328 Authors: Žákovská A, Janeček J, Nejezchlebová H, Kučerová HL Abstract INTRODUCTION AND OBJECTIVES: The existence of a blood group preference for ticks is a problem widely discussed among the lay public but often neglected by the scientific community. The Ixodes ricinus tick transmits serious zoonotic diseases such as Lyme borreliosis, tick encephalitis, or anaplasmosis. The preventive strategies include vaccinatio...
Source: Annals of Agricultural and Environmental Medicine : AAEM - Category: Environmental Health Authors: Tags: Ann Agric Environ Med Source Type: research
The objectives of this study were to use data from client-owned cats in an Ixodes scapularis endemic area to evaluate for clinical associations with diagnostic test results for Anaplasma phagocytophilum and Borrelia burgdorferi and to provide information from a group of cats with possible borreliosis as the cause of clinical manifestations of disease. All cases were evaluated at one clinic, medical records were evaluated, and sera from all cats were tested using one of two commercially available assays labeled for the use with dog sera (SNAP ® 4Dx® or SNAP® 4Dx® Plus; IDEXX Laboratories, Westbrook, ME).
Source: Topics in Companion Animal Medicine - Category: Veterinary Research Authors: Tags: Research Article Source Type: research
For most of us, springtime marks the return of life to a dreary landscape, bringing birdsong, trees in bud, and daffodils in bloom. But if you work for the Centers for Disease Control and Prevention (CDC), the coming of spring means the return of nasty diseases spread by ticks and mosquitoes. The killjoys at CDC celebrated the end of winter with a bummer of a paper showing that infections spread by ticks doubled in the United States from 2004 to 2016. (Tick populations have exploded in recent decades, perhaps due to climate change and loss of biodiversity.) Lyme disease The most common infection spread by ticks in the US i...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Health Infectious diseases Source Type: blogs
This article originally appeared on Health.com
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized healthytime onetime public health Source Type: news
The following data are abstracted from Gideon and the Gideon e-book series. [1]  Charts were created using an interactive tool driven by over 30,000 base graphs in the program. [2] Chart 1 contrasts trends for reported incidence of Lyme disease and Rocky Mountain spotted fever (RMSF).  Note that while rates of Lyme disease in 2016 are 15-fold those reported in 1987, those of RMSF increased by a factor of seven.  The number of fatal cases for both diseases have remained similar in recent years (i.e., the relative case-fatality rate of Lyme disease has decreased) Chart 2 summarizes incidence data for a variet...
Source: GIDEON blog - Category: Databases & Libraries Authors: Tags: Ebooks Epidemiology Graphs ProMED Source Type: blogs
Abstract Anaplasmosis, cat-scratch disease, and Lyme disease are emerging vector-borne infectious diseases in Korea. Although the prevalence of vector-borne pathogens (VBPs) in domestic animals and vector arthropods has been documented, there is limited information on the presence of VBPs in wild animals. The raccoon dog (Nyctereutes procyonoides), a wild canid found in East Asia and Europe, represents a potential wildlife reservoir for zoonotic diseases. To investigate the prevalence of VBPs in raccoon dogs, 142 carcasses and 51 blood samples from captured raccoon dogs were collected from 2003 to 2010 and from 20...
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research
Publication date: Available online 31 January 2018 Source:Ticks and Tick-borne Diseases Author(s): Lars Eisen The blacklegged tick, Ixodes scapularis, is the primary vector to humans in the eastern United States of the deer tick virus lineage of Powassan virus (Powassan virus disease); the protozoan parasite Babesia microti (babesiosis); and multiple bacterial disease agents including Anaplasma phagocytophilum (anaplasmosis), Borrelia burgdorferi and Borrelia mayonii (Lyme disease), Borrelia miyamotoi (relapsing fever-like illness, named Borrelia miyamotoi disease), and Ehrlichia muris eauclairensis (a minor causative age...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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