The oligopeptide ABC transporter OppA4 negatively regulates the virulence factor OspC production of the Lyme disease pathogen

In this study, possible role of another Oligopeptide ABC transporter, OppA4 in ospC expression was investigated by construction of an oppA4 deletion mutant and the complemented strain. Inactivation of oppA4 resulted an increased production of OspC, suggesting that OppA4 has a negative impact on ospC expression. Expression of ospC is controlled by Rrp2-RpoN-RpoS, the central pathway essential for mammal infection. We showed that increased ospC expression in the oppA4 mutant was due to an increased rpoS expression. We then further investigated how OppA4 negatively regulates this pathway. Two regulators, BosR and BadR, are known to positively and negatively, respectively, regulate the Rrp2-RpoN-RpoS pathway. We found that deletion of oppA4 resulted in an increased level of BosR. Previous reports showed that bosR is mainly regulated at the post-transcriptional level by other factors. However, OppA4 appears to negatively regulate bosR expression at the transcriptional level. The finding of OppA4 involved in regulation of the Rrp2-RpoN-RpoS pathway further reinforces the importance of nutritional virulence to the enzootic cycle of B. burgdorferi.
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research

Related Links:

This study aimed to investigate the presence and perform molecular characterization of Anaplasmataceae, Rickettsia spp., and Borrelia burgdorferi sensu lato (sl) in tick species collected in Corsica. Ticks from cattle (Bos taurus), sheep (Ovis aries), and rodents (Rattus rattus) were collected from May to September 2016. DNA was purified from ticks, submitted to quantitative real-time polymerase chain reaction (qPCR) and sequenced for phylogenetic analysis. In total, 660 ticks were collected from 111 animals during the study. The most abundant collected tick species from cattle was Rhipicephalus bursa (n = 49...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Authors: Boulanger N, Boyer P, Talagrand-Reboul E, Hansmann Y Abstract Ticks are a major group of arthropod vectors, characterized by the diversity of pathogens they transmit, by their impact on human and animal health, and by their socioeconomic implication especially in countries of the Southern Hemisphere. In Europe, Ixodes is the most important tick due to its wide distribution in the ecosystems and the variety of transmitted pathogens, in particular Borrelia (responsible for Lyme borreliosis), but also the tick-borne encephalitis virus. Their increased presence in the environment since the beginning of the 20t...
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research
Purpose: The South Bohemian Region of the Czech Republic is an endemic area of tick-borne encephalitis (TBE). In 2017, the inhabitants of Římov municipality developed a serious course of TBE and Lyme borreliosis (LB). This led experts to research and serology examinations of the inhabitants of Římov in order to detect the seroprevalence of TBE and LB.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: 21.130 Source Type: research
Purpose: In southern Canada, Lyme disease (LD) is an emerging tick-borne disease caused by Borrelia burgdorferi. Cases have increased from 144 to 987 between 2009 and 2016. The infection is more frequent in children and older adults probably due to increased exposure to high risk areas during outdoor activities. The aim of this study is to describe the clinical presentation and course of illness among children reported in Monteregie, an emerging area from Quebec province located north of New York state.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: 21.166 Source Type: research
Publication date: Available online 19 January 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Isis Arsnoe, Jean I. Tsao, Graham J. HicklingAbstractMost people who contract Lyme borreliosis in the eastern United States (US) acquire infection from the bite of the nymphal life stage of the vector tick Ixodes scapularis, which is present in all eastern states. Yet
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 12 January 2019Source: Ticks and Tick-borne DiseasesAuthor(s): William J. Landesman, Kenneth Mulder, Brian F. Allan, Laura A. Bashor, Felicia Keesing, Kathleen LoGiudice, Richard S. OstfeldAbstractTick microbiomes may play an important role in pathogen transmission. However, the drivers of microbiome variation are poorly understood, and this limitation has impeded mechanistic understanding of the functions of microbial communities for pathogen acquisition. The goal of this research was to characterize the role of the blood meal host in structuring the microbiome of Ixodes scapularis, the ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
In this study, we investigated tick-borne bacterial pathogens in wild boar, hare, and fox and their ticks in Turkey. A total of 102 tick pools comprised of 445 ticks and blood samples were analyzed for the presence of bacterial DNA by PCRs targeted rickettsial gltA and ompA genes, 5S-23S rDNA gene for Borrelia spp., and msp4 gene for Anaplasma spp. As a result of PCR and sequence analyses, three pathogenic spotted fever group (SFG) rickettsiae, two SFG rickettsiae with unknown pathogenicity and one pathogenic Borrelia burgdorferi sensu lato were detected in samples obtained from wild animals. Rickettsia slovaca was detecte...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
This study aims to estimate the incidence of the different clinical manifestations of LB in Belgium. The incidence of erythema migrans (EM) was estimated through the network of sentinel general practices at 97.6/100,000 inhabitants (uncertainty interval [UI] 82.0–113.0) for the period 2015 to 2017. This result was used to estimate the incidence of other LB manifestations based on their proportional distribution (ratios) to EM reported in the neighboring countries of Belgium. To estimate these ratios, we performed a systematic review of studies published between February 1, 2008 and January 31, 2018 and pooled the res...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 23 December 2018Source: Ticks and Tick-borne DiseasesAuthor(s): Amandine Boeuf, Gilles Schnell, Quentin Bernard, Aurelie Kern, Benoît Westermann, Laurence Ehret-Sabatier, Antoine Grillon, Frederic Schramm, Benoît Jaulhac, Nathalie BoulangerAbstractUnderstanding the mechanism of pathogen transmission is essential for the development of strategies to reduce arthropod-borne diseases. The pharmaco- and immunomodulatory properties of insect and acarine saliva play an essential role in the efficiency of pathogen transmission. The skin as the site where arthropod saliva and pathogens...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 14 December 2018Source: Ticks and Tick-borne DiseasesAuthor(s): George Psevdos, Teresa Khoo, Robert Chow, Christopher L Romano, Scott CampbellAbstractIn North America, Lyme disease (LD) is caused predominantly by the spirochete Borrelia burgdorferisensu stricto, and is transmitted by blacklegged ticks. Long Island, New York, is highly endemic for the disease. The C6 peptide (C6P) is currently used as a screening test for LD in our institution. Our objective was to examine how screening with C6P concorded with diagnosis of LD at the Veterans Affairs Medical Center, Northport, Long Island. ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
More News: Lyme Disease | Nutrition | Study | Tickborne Diseases | Zoology