CXCL13 concentrations in cerebrospinal fluid of patients with Lyme neuroborreliosis and other neurological disorders determined by Luminex and ELISA

Publication date: July 2018Source: Ticks and Tick-borne Diseases, Volume 9, Issue 5Author(s): Mateusz Markowicz, Anna-Margarita Schötta, Michael Kundi, Petra Bogovič, Katarina Ogrinc, Franc Strle, Gerold StanekAbstractThe aims of the study were to determine and compare the concentration of CXCL13 in cerebrospinal fluid (CSF) of patients with Lyme neuroborreliosis (LNB) and various other neurological disorders applying a Luminex based assay and ELISA, and to find factors associated with CXCL13 concentration. CSF samples obtained from four clinically well-defined groups of patients (proven LNB, suspected LNB, tick-borne encephalitis (TBE), and aseptic meningitis/meningoencephalitis other than TBE) – 25 samples per group – were analyzed. The performance of the Luminex recomBead CXCL13 assay (Microgen, Neuried, Germany) and ELISA (Euroimmun, Lübeck, Germany) was assessed by receiver operating characteristics. CXCL13 cut-off values were presented as functions of CSF lymphocyte/monocyte counts. Demographic variables, CSF findings, and history of erythema migrans were assessed as possible predictors for CXCL13 CSF concentrations by a general linear model. The calculated cut-off values determined by the maximum of the Youden index were>131 pg/mL for recomBead and>259 pg/mL for the ELISA. RecomBead showed a sensitivity of 88% (68.8–97.5%) and a specificity of 94% (83.5–98.7%). For the ELISA the corresponding values were 84% (63.9–95.5...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research

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Publication date: Available online 16 April 2019Source: Best Practice &Research Clinical Endocrinology &MetabolismAuthor(s): German Rubinstein, Andrea Osswald, Stephanie Zopp, Katrin Ritzel, Marily Theodoropoulou, Felix Beuschlein, Martin ReinckeAbstractCONTEXT:Cushing’s disease (CD) is the most common etiology of Cushing’s syndrome (CD) due to corticotroph pituitary adenoma, which are in most cases small (80-90% microadenomas) and in about 40% cannot be visualized on imaging of the sella. First-line treatment for CD is transsphenoidal surgery (TSS) with the aim of complete adenoma removal and preservat...
Source: Best Practice and Research Clinical Endocrinology and Metabolism - Category: Endocrinology Source Type: research
Hongye Zhao1,2, Tiezheng Zheng1, Xiaohan Yang1, Ming Fan3, Lingling Zhu3, Shuhong Liu3, Liying Wu3 and Changkai Sun1,4* 1Department of Physiology and Key Laboratory of Brain Diseases of Liaoning Province, School of Basic Medical Sciences, Dalian Medical University, Dalian, China 2Department of Physiology, School of Basic Medical Sciences, Qiqihar Medical University, Qiqihar, China 3Department of Brain Protection and Plasticity, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing, China 4Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineerin...
Source: Frontiers in Neurology - Category: Neurology Source Type: research
In this study, the StCas9 derived from Streptococcus thermophilus together with the Drosha-mediated sgRNA-shRNA structure were combined to boost the G to A base editing on the IGF2 SNP site, which we called “SNP editing.” The codon-humanized StCas9 as we previously reported was firstly compared with the prevalently used SpCas9 derived from Streptococcus pyogenes using our idiomatic surrogate report assay, and the StCas9 demonstrated a comparable targeting activity. On the other hand, by combining shRNA with sgRNA, simultaneous gene silencing and genome targeting can be achieved. Thus, the novel IGF2.sgRNA-LIG4....
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Conclusion: This case of thyroid bed PG supports the idea that PG reflects a cutaneous phenotype encompassed in the spectrum of systemic neutrophilic diseases. Endocrinologists should be aware that thyroid bed PG involvement is an albeit rare differential diagnosis to consider in patients who had undergone thyroid surgery, especially with a history of PG. Introduction Papillary thyroid carcinoma (PTC), the main variant of differentiated thyroid carcinoma (DTC), is the most common thyroid cancer, accounting for 70–80% of all thyroid carcinomas and occurring predominantly in women, generally with a good prognos...
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
Tatsuya Daikoku1,2* and Masato Yumoto1 1Department of Clinical Laboratory, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan 2Department of Neuropsychology, Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany In an auditory environment, humans are frequently exposed to overlapping sound sequences such as those made by human voices and musical instruments, and we can acquire information embedded in these sequences via attentional and nonattentional accesses. Whether the knowledge acquired by attentional accesses interacts with that acquired by nonattentional accesses is...
Source: Frontiers in Human Neuroscience - Category: Neuroscience Source Type: research
Therapeutic Targeting of Fibrotic Epithelial-Mesenchymal Transition–An Outstanding Challenge Attila Fintha1, Ákos Gasparics2, László Rosivall3 and Attila Sebe3,4* 12nd Department of Pathology, Semmelweis University, Budapest, Hungary 21st Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary 3Department of Pathophysiology, International Nephrology Research and Training Center, Semmelweis University, Budapest, Hungary 4Division of Medical Biotechnology, Paul Ehrlich Institute, Langen, Germany Back in 1995, a landmark paper was published, which shaped the fi...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
CONCLUSION: Our findings illustrate the importance of a qualitative approach by analyzing physical activity resources. Concerning the quality of playgrounds, the data from this study support the deprivation amplification hypothesis, meaning that children who are already socially disadvantaged might experience a further contextual disadvantage. PMID: 30987509 [PubMed - as supplied by publisher]
Source: Journal of Physical Activity and Health - Category: Sports Medicine Tags: J Phys Act Health Source Type: research
CONCLUSION: This case report is the first documented instance of hemorrhagic encephalitis identified in a patient with NMO. Among the manifestations of brain involvement in patients with NMO, hemorrhagic encephalitis is rare but can be observed. PMID: 30985265 [PubMed - as supplied by publisher]
Source: Journal of Spinal Cord Medicine - Category: Orthopaedics Tags: J Spinal Cord Med Source Type: research
Researchers from the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, the University of Trento in Italy, and other international collaborators leveraged a machine learning algorithm to identify a subset of gut bacteria associated ...
Source: Medgadget - Category: Medical Devices Authors: Tags: Genetics GI Informatics Oncology Pathology Source Type: blogs
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