Polymorphonuclear cells and reactive oxygen species in contagious bovine pleuropneumonia: New insight from in vitro investigations

Publication date: July 2018Source: Veterinary Immunology and Immunopathology, Volume 201Author(s): Giovanni Di Teodoro, Giuseppe Marruchella, Francesco Mosca, Andrea Di Provvido, Flavio Sacchini, Pietro Giorgio Tiscar, Massimo ScacchiaAbstractReactive oxygen species (ROS) are suggested to play a role in the pathogenesis of contagious bovine pleuropneumonia, a severe respiratory disorder caused by Mycoplasma mycoides subsp. mycoides (Mmm). The present study investigated the generation of ROS by different strains of Mmm, as well as their effect on the oxidative response of bovine neutrophils. The production of ROS was indirectly measured using a luminol-based chemiluminescence assay. Our results confirm that Mmm can produce ROS via the metabolism of glycerol, significant differences existing between African and European strains. Mmm was capable of adhering to the external surface of neutrophils. Interestingly, Mmm enhanced the respiratory burst of bovine neutrophils. This activity was particularly pronounced with the African field strain and in presence of glycerol. Taken together, our data argue in favour of a major role for neutrophils as the main source of ROS in contagious bovine pleuropneumonia.
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research