Delivery of MGMT mRNA to glioma cells by reactive astrocyte-derived exosomes confers a temozolomide resistance phenotype

In this study, we show that glioma cells stimulate normal human astrocyte (NHA) into reactive astrocyte (RAS) in a non-contact manner. Additionally, the amount of O6-alkylguanine DNA alkyltransferase (MGMT) mRNA in exosomes (EXOs) released by RAS was significantly increased compared with that in non-reactive NHA. Importantly, MGMT-negative glioma cells can take up RAS-EXOs and acquire a temozolomide (TMZ)-resistant phenotype via the translation of exogenous exosomal MGMT mRNA both in vitro and in vivo.

Source: Cancer Letters - July 6, 2018 Category: Cancer & Oncology Authors: Tianfu Yu, XieFeng Wang, Tongle Zhi, Junxia Zhang, Yingyi Wang, Er Nie, Fengqi Zhou, Yongping You, Ning Liu Tags: Original Articles Source Type: research