GSE116701 Analysis of Wild Type and Leptin-overexpressing Pig PBMC Transcriptomes

Contributors : Xiang Chen ; Hongjiang WeiSeries Type : Expression profiling by high throughput sequencingOrganism : Sus scrofaPurpose: To find the difference between leptin-overexpressing and wt pig.Methods: Peripheral blood mononuclear cells (PBMCs) mRNA profiles of 6-month-old wild-type (WT) and leptin-overexpressing pig were generated by deep sequencing. Sequencing libraries were constructed according to the protocol for the Illumina TruSeq Sample preparation kit. Sequencing was performed on the Illumina X Ten sequencer. Library construction and sequencing were performed at the Genergy Biotech Co., Ltd. (Shanghai).Results: RNA-seq was performed to detect the transcriptomic changes in PBMCs in the transgenic leptin pigs. With 2-folds change and p
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Sus scrofa Source Type: research