Identification of antioxidant constituents of the aerial part of Plantago asiatica using LC–MS/MS coupled DPPH assay

Publication date: August 2018Source: Phytochemistry Letters, Volume 26Author(s): Jong Hoon Ahn, Yang Hee Jo, Seon Beom Kim, Ayman Turk, Kyung-Eon Oh, Bang Yeon Hwang, Ki Yong Lee, Mi Kyeong LeeAbstractScreening of EtOAc soluble fraction of Plantago asiatica (Plantaginaceae) using HPLC–MS/MS coupled DPPH online assay suggested the presence of antioxidant compounds, which characterized as phenylethanoid glycosides. Twenty-nine compounds including three new compounds, plantalides A–C, a phenylethanoid (1), a phenolic compound (9) and an iridoid (10), were isolated from P. asiatica. Consistent with HPLC–MS/MS analysis, seven phenylethanoid glycosides (1–7) including a new phenylethanoid glycoside (1) showed antioxidant with ranging IC50 values of 22.9–88.5 μM. These results suggested that phenylethanoid glycosides were contributed to the antioxidant activity of P. asiatica and HPLC–MS/MS coupled online assay is powerful tool for predicting active compounds with short time and limited amounts.Graphical abstract

Source: Phytochemistry Letters - July 6, 2018 Category: Chemistry Source Type: research