Generation of a human induced pluripotent stem cell line, KSCBi003-A, from human adipose tissue-derived mesenchymal stem cells using a chromosomal integration-free system

Publication date: August 2018Source: Stem Cell Research, Volume 31Author(s): Hye Young Choi, So-Jung Kim, Gue Youn Go, Ara Kwon, Young Sam Im, Hye-Yeong Ha, Jin Tae Hong, Ji-won Jung, Soo Kyung KooAbstractWe generated a human induced pluripotent stem cell (hiPSC) line, KSCBi003-A, from adipose tissue-derived mesenchymal stem cells (Ad-MSCs) using a Sendai virus-based gene delivery system. We confirmed that the KSCBi003-A has a normal karyotype and short tandem repeat (STR)-based identities that match the parent cells. We also confirmed that the cell line expresses pluripotent stem cell markers such as Nanog, OCT4, SSEA-4, TRA-1-60, and TRA-1-81. We also analyzed that the KSCBi003-A has an ability to differentiate three germ layers (ectoderm, mesoderm, endoderm). This cell line is registered and available at the National Stem Cell Bank, Korea National Institute of Health.
Source: Stem Cell Research - Category: Stem Cells Source Type: research