Differentiation of neural crest stem cells from nasal mucosa into motor neuron-like cells

Publication date: October 2018Source: Journal of Chemical Neuroanatomy, Volume 92Author(s): Zohreh Bagher, Seyed Kamran Kamrava, Rafieh Alizadeh, Mohammad Farhadi, Moloud Absalan, Masoumeh Falah, Faezeh Faghihi, Arash Zare-Sadeghi, Ali KomeiliAbstractCell transplantation is a potential therapeutic approach for repairing neuropathological and neurodegenerative disorders of central nervous system by replacing the degenerated cells with new ones. Among a variety of stem cell candidates to provide these new cells, olfactory ectomesenchymal stem cells (OE-MSCs) have attracted a great attention due to their neural crest origin, easy harvest, high proliferation, and autologous transplantation. Since there is no report on differentiation potential of these cells into motor neuron-like cells, we evaluated this potential using Real-time PCR, flowcytometry and immunocytochemistry after the treatment with differentiation cocktail containing retinoic acid and Sonic Hedgehog. Immunocytochemistry staining of the isolated OE-MSCs demonstrated their capability to express nestin and vimentin, as the two markers of primitive neuroectoderm. The motor neuron differentiation of OE-MSCs resulted in changing their morphology into bipolar cells with high expression of motor neuron markers of ChAT, Hb-9 and Islet-1 at the level of mRNA and protein. Consequently, we believe that the OE-MSCs have great potential to differentiate into motor neuron-like cells and can be an ideal stem cell source for the t...
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research