Biosynthesis of zinc oxide nanoparticles usingMangifera indica leaves and evaluation of their antioxidant and cytotoxic properties in lung cancer (A549) cells

Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): S. Rajeshkumar, S. Venkat Kumar, Arunachalam Ramaiah, Happy Agarwal, T. Lakshmi, Selvaraj Mohana RoopanAbstractGreen synthesis is an eco-friendly approach to nanoparticle production, which eliminates the use of toxic chemicals, high temperatures, and costly equipment needed for traditional physical and chemical synthesis methods. This eco-friendly approach was used in the present study to biosynthesize zinc oxide nanoparticles (ZnO NPs) from Mangifera indica (mango) leaves which were then evaluated for their antioxidant activity and cytotoxic effects on lung cancer A549 cells. Synthesized ZnO nanoparticles were characterized using UV–vis spectroscopy, XRD, SEM, and EDX analyses. The XRD and SEM analyses showed 45–60 nm as the size of synthesized nanoparticles, the pure crystal form of ZnO NPs, and the shape of the NPs as nearly spherical and hexagonal quartzite. The antioxidant potential of nanoparticles was estimated using a DPPH free radical scavenging assay. The percent of viable cells was inversely proportional to the concentration of ZnO nanoparticles at 25 μg/mL concentration. The MTT assay used for cytotoxicity evaluation depicted the significant cytotoxic effect of ZnO NPs against the A549 lung cancer cell line. The drop in the proportion of viable A549 cells after exposure to ZnO NPs was comparable to the effects of the standard drug used i.e. cyclophosphamide. Antiox...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research