GSE114410 DNA topoisomerase and supercoil accumulation across yeast genome

Contributors : Yathish J Achar ; Mohamood Adhil ; Marco FoianiSeries Type : Genome binding/occupancy profiling by genome tiling arrayOrganism : Saccharomyces cerevisiaeDNA toposiomserases assist DNA replication& transcription events by controlling supercoiling alterations. We investigated supercoil distribution across yeast genome and compared with accumulation of RNA pol2 and DNA topoisomerases particularly in S-phase. Our data indicate that Top2 along with Hmo1 maintain negative supercoil at gene boundaries by stabilizing alternative DNA structures.To understand how DNA super helical tension accumulate across the genome we have adopted previously described method [Naughton C et al., 2013] to budding yeast where a biotin molecule was attached to TMP via a linker (bTMP). The Chip on chip analysis for proteins was carried out as described (Bermejo R et al., 2009). For RNA-DNA hybrids DRIP-chip is carried out as described previously (Chan YA et al., 2014). Supercoiled regions are then compared with RNA pol2 (RPB3-chip), DNA Topoisomerase (Top1-chip)& RNA-DNA hybrid (DRIP-chip) .

Source: GEO: Gene Expression Omnibus - July 2, 2018 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by genome tiling array Saccharomyces cerevisiae Source Type: research