GSE116500 Spec-seq unveils transcriptional subpopulations of antibody-secreting cells following influenza vaccination

Contributors : Karlynn E Neu ; Min Huang ; Jennifer La ; Marcos C Vieira ; Kangchon Kim ; Nai-Ying Zheng ; Mario Cortese ; Karla T Rojas ; Carole Henry ; Dustin Shaw ; Charles L Dulberger ; Bali Pulendran ; Sarah Cobey ; Aly A Khan ; Patrick C WilsonSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensVaccines are among the most effective public health tools for combating certain infectious diseases such as influenza. The role of the humoral immune system in vaccine-induced protection is widely appreciated; however, our understanding of how antibody specificities relate to B cell function remains limited, due to the complexity of polyclonal antibody responses. To address this, we developed the Spec-seq framework, which allows for simultaneous monoclonal antibody (mAb) characterization and transcriptional profiling from the same single cell. Here, we present the first application of the Spec-seq framework, which we applied to human plasmablasts after influenza vaccination in order to characterize transcriptional differences governed by B cell receptor (BCR) isotype and vaccine reactivity. Our analysis did not find evidence of long-term transcriptional specialization between plasmablasts of different isotypes. However, we did find enhanced transcriptional similarity between clonally related B cells, as well as distinct transcriptional signatures ascribed by BCR vaccine recognition. These data suggest IgG and IgA vaccine positive plasma...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research