GSE112212 Nucleotide resolution genome-wide mapping of 8-oxoG damage

Contributors : Junzhou Wu ; Shana J SturlaSeries Type : OtherOrganism : Saccharomyces cerevisiae BY4741Single nucleotide resolution sequencing of DNA damage is required to decipher the complex causal link between the identity and location of DNA adduct and mutation patterns in the genome. We coupled the specificity of the repair enzyme with the efficiency of a one-pot click-DNA ligation reaction to insert a readable oligonucleotide code sequence. The biocompatible code enabled high-throughput, base resolution sequencing of the 8-oxoguanine site and thus have named the method click-code-seq. By applying click-code-seq to a eukaryotic (yeast) genome, we uncovered thousands of 8-oxoguanine sites with features and patterns suggesting a potential relationship to chromatin formation and transcription.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Other Saccharomyces cerevisiae BY4741 Source Type: research