Actin-binding LIM Protein 1 Regulates Receptor Activator of NF- κB Ligand-Mediated Osteoclast Differentiation and Motility.

Actin-binding LIM Protein 1 Regulates Receptor Activator of NF-κB Ligand-Mediated Osteoclast Differentiation and Motility.
BMB Rep. 2018 Jun 20;:
Authors: Jin SH, Kim H, Gu DR, Park KH, Lee YR, Choi Y, Lee SH
Abstract
Actin-binding LIM protein 1 (ABLIM1), a member of the LIM-domain protein family, mediates interactions between actin filaments and cytoplasmic targets. However, the role of ABLIM1 in osteoclast and bone metabolism has not been reported. In the present study, we investigated the role of ABLIM1 in the receptor activator of NF-κB ligand (RANKL)-mediated osteoclastogenesis. ABLIM1 expression was induced by RANKL treatment and knockdown of ABLIM1 by retrovirus infection containing Ablim1-specific short hairpin RNA (shAblim1) decreased mature osteoclast formation and bone resorption activity in a RANKL-dose dependent manner. Coincident with the downregulated expression of osteoclast differentiation marker genes, the expression levels of c-Fos and the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), critical transcription factors of osteoclastogenesis, were also decreased in shAblim1-infected osteoclasts during RANKL-mediated osteoclast differentiation. In addition, the motility of preosteoclast was reduced by ABLIM1 knockdown via modulation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt/Rac1 signaling pathway, suggesting another regulatory mechanism of ABLIM1 in osteoclast formation. These data ...

Source: BMB Reports - June 20, 2018 Category: Biochemistry Authors: Jin SH, Kim H, Gu DR, Park KH, Lee YR, Choi Y, Lee SH Tags: BMB Rep Source Type: research