Regulation of Renal Na Transporters in Response to Dietary K.

Regulation of Renal Na Transporters in Response to Dietary K. Am J Physiol Renal Physiol. 2018 Jun 20;: Authors: Yang L, Xu S, Guo X, Uchida S, Weinstein AM, Wang T, Palmer LG Abstract Changes in the expression of Na transport proteins were measured in the kidneys of mice with increased dietary K intake for 1 week. The epithelial Na channel (ENaC) was upregulated, with enhanced expression of full-length and cleaved forms of αENaC and cleaved γENaC. At the same time, the amount of the NaCl cotransporter (NCC) and its phosphorylated form decreased by ~50% and ~80% respectively. The expression of the phosphorylated form of the Na-K-2Cl cotransporter NKCC2 also decreased, despite an increase in overall protein content. The effect was stronger in males (80%) than in females (40%). This implies that less Na+ is reabsorbed in the TALH and DCT along with Cl-, while more is reabsorbed in the aldosterone-sensitive distal nephron in exchange for secreted K+. The abundance of the proximal tubule Na/H exchanger NHE3 decreased by ~40%, with similar effects in males and females. Time-course studies indicated that NCC and NHE3 protein decreased progressively over 7 days on a high-K diet. Expression of mRNA encoding these proteins increased, implying that the decreased protein levels resulted from decreased rates of synthesis or increased rates of degradation. The potential importance of changes in NHE3, NKCC2 and NCC in promoting K+ excretion was ...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research