Molecules, Vol. 23, Pages 1490: Metabolomic Analysis of Biochemical Changes in the Serum and Urine of Freund ’s Adjuvant-Induced Arthritis in Rats after Treatment with Silkworm Excrement

Molecules, Vol. 23, Pages 1490: Metabolomic Analysis of Biochemical Changes in the Serum and Urine of Freund’s Adjuvant-Induced Arthritis in Rats after Treatment with Silkworm Excrement Molecules doi: 10.3390/molecules23061490 Authors: Tianyao Zheng Shulan Su Xinxin Dai Liwen Zhang Jin-Ao Duan Zhen Ou-Yang Silkworm excrement (SE), is used as a traditional antirheumatic medicine in China. The present study was designed to investigate the therapeutic efficacy of water fraction of SE (ST) and ethanol fraction of SE (CT) at two different doses on adjuvant induced arthritis (AA) rats. Arthritis severity was evaluated by body weight, paw thickness, histological changes and index of paws oedema and spleen. Serum samples were collected for estimation of biochemical indicators and cytokines. In addition, a metabonomic method based on the ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) had been established to investigate the holistic efficacy of SE by serum and urine. Multivariate statistical approaches, such as partial least-squares discriminant analysis (PLS-DA) and orthogonal projection to latent structures squares-discriminant analysis (OPLS-DA) were built to evaluate the therapeutic effects of SE and find potential biomarkers and metabolic pathways. Administration with SE significantly ameliorated the AA severity, including body weight loss, paw swelling, histological changes and the levels of bio...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research