Inhibition of LPS-induced inflammatory responses by Satureja hortensis extracts in J774.1 macrophages.

Inhibition of LPS-induced inflammatory responses by Satureja hortensis extracts in J774.1 macrophages.
J Immunoassay Immunochem. 2018 Jun 18;:1-18
Authors: Farzaneh Z, Kalantar K, Iraji A, Amirghofran Z
Abstract
Macrophages are among the main cells involved in generation of inflammation. To investigate the anti-inflammatory effect of Satureja hortensis (summer savory), lipopolysaccharide (LPS)-activated J774.1 macrophages were treated with various extracts, and the expression and release of various inflammatory molecules by macrophages were examined. We showed that dichloromethane and hexane extracts reduced nitric oxide (NO) production more efficiently than other extracts. Both extracts decreased gene expression of inducible NO synthase (iNOS) (<0.44 fold of control), cyclooxygenase (COX)-2 (<0.29 fold), interleukin (IL)-1β (<0.41 fold), IL-6 (<0.25 fold) and tumor necrosis factor (TNF)-α (<0.2 fold). The extracts reduced IL-6 and IL-1β proteins production from macrophages. Surface intensity of expression of intercellular adhesion molecule (ICAM)-1 decreased to 845 ± 28.1 (dichloromethane) and 715 ± 48.6 (hexane) compared to the control (902 ± 73.1). These findings showed that Satureja hortensis, by influencing macrophages and related mediators, could contribute to reduction of inflammation and might be useful as an anti-inflammatory agent.
PMID: 29913095 [PubMed - as supplied by publisher]

Source: Journal of Immunoassay and Immunochemistry - June 20, 2018 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research