Optimization of subculture and DNA extraction steps within the whole genome sequencing workflow for source tracking of Salmonella enterica and Listeria monocytogenes

Publication date: Available online 18 June 2018 Source:Journal of Microbiological Methods Author(s): Johan Gimonet, Anne-Catherine Portmann, Coralie Fournier, Leen Baert This work shows that an incubation time reduced to 4–5 h to prepare a culture for DNA extraction followed by an automated DNA extraction can shorten the hands-on time, the turnaround time by 30% and increase the throughput while maintaining the WGS quality assessed by high quality Single Nucleotide Polymorphism analysis.
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research