Hyporientalin A, an anti- Candida peptaibol from a marine Trichoderma orientale

AbstractATrichoderma orientale strain LSBA1 was isolated from the Mediterranean marine spongeCymbaxinella damicornis. The crude extract ofT. orientale mycelium showed inhibitory activity against growth of Gram-positive and Gram-negative bacteria as well as clinical isolates ofCandida albicans. Purification of the anti-Candida component was performed using a combination of open silica gel-60 column and reverse phase high performance liquid chromatography. The active compound called hyporientalin A has been identified as a peptaibol analogue of longibrachin-A-II using mass spectrometry. It exhibited fungicidal activity against clinical isolates ofC. albicans with minimal inhibitory concentrations (MICs) ranging from 2.49 to 19.66  µM, comparable to that of the antifungal agent amphotericin B. Our data support the use of hyporientalin A as a promising new and efficient antifungal drug in the treatment of candidiasis while controlling toxicity.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research