Non-Invasive Immunofluorescence Assessment of Lycopene Supplementation Status in Skin Smears.

Non-Invasive Immunofluorescence Assessment of Lycopene Supplementation Status in Skin Smears. Monoclon Antib Immunodiagn Immunother. 2018 Jun 14;: Authors: Petyaev IM, Zigangirova NA, Pristensky D, Chernyshova M, Tsibezov VV, Chalyk NE, Morgunova EY, Kyle NH, Bashmakov YK Abstract Circulating lycopene level is negatively associated with the prevalence of cardiovascular disease, cancers (prostate and breast), type 2 diabetes mellitus, and aging. Traditionally, lycopene is measured in biological specimens by a combination of high-performance liquid chromatography (HPLC) and mass spectrometry methods. Moreover, as we recently reported, tissue/cell lycopene depositions can be observed by the immunohistochemistry method with a newly developed monoclonal antibody (mAb) against lycopene. A main objective of this study is to evaluate the performance of a new noninvasive immunofluorescence (IF) lycopene quantification skin test with mAbs against lycopene versus HPLC lycopene assay of serum lycopene in volunteers subjected to lycopene supplementation and represents its novelty. For this purpose, 32 healthy volunteers, 30-40 years old, were supplemented with lycopene (nā€‰=ā€‰15) or placebo (nā€‰=ā€‰17) for a period of 4 weeks. It was found that lycopene supplementation leads to a significant increase in serum lycopene concentration after 2 and 4 weeks by 2.6- and 3.4-fold over control, respectively. This was accompanied by a concordant step-wi...
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research