Nanoscale polyelectrolyte complexes encapsulating mRNA and long-chained siRNA for combinatorial cancer gene therapy

Publication date: 25 August 2018 Source:Journal of Industrial and Engineering Chemistry, Volume 64 Author(s): Myung Goo Kim, Sung Duk Jo, Ji Hoon Jeong, Sun Hwa Kim To precisely regulate target genes that are abnormally expressed in cancers, we suggest an RNA-mediated multigene targeting system that co-encapsulates siRNA against vascular endothelial growth factor (VEGF) and mRNA encoding phosphatase and tensin homolog (PTEN). Polymerized long-chain siRNAs (L-siRNAs) formed stable and condensed nanocomplexes with mRNAs using thiolated glycol chitosans (tGCs) as gene carriers. The mRNA/L-siRNA/tGC nanocomplexes (MSNs) exhibited efficient intracellular delivery and superior anti-tumor efficiency with simultaneous up and down-regulation of PTEN and VEGF, respectively. The MSN system can be considered as a new platform for cancer gene therapy requiring accurate control of multiple gene expressions. Graphical abstract
Source: Journal of Industrial and Engineering Chemistry - Category: Chemistry Source Type: research