An SPR based immunoassay for the sensitive detection of the soluble epithelial marker E-cadherin

Publication date: Available online 15 June 2018 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Daniele Vergara, Monica Bianco, Rosanna Pagano, Paola Priore, Paola Lunetti, Flora Guerra, Simona Bettini, Sonia Carallo, Alessandra Zizzari, Elena Pitotti, Livia Giotta, Loredana Capobianco, Cecilia Bucci, Ludovico Valli, Michele Maffia, Valentina Arima, Antonio Gaballo Protein biomarkers are important diagnostic tools for cancer and several other diseases. To be validated in a clinical context, a biomarker should satisfy some requirements including the ability to provide reliable information on a pathological state by measuring its expression levels. In parallel, the development of an approach capable of detecting biomarkers with high sensitivity and specificity would be ideally suited for clinical applications. Here, we performed an immune-based label free assay using Surface Plasmon Resonance (SPR)-based detection of the soluble form of E-cadherin, a cell–cell contact protein that is involved in the maintaining of tissue integrity. With this approach, we obtained a specific and quantitative detection of E-cadherin from a few hundred μl of serum of breast cancer patients by obtaining a 10-fold enhancement in the detection limit over a traditional colorimetric ELISA. Graphical abstract
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research