Aberrant expression of FoxP3 in a human T  cell line possessing regulatory T cell‑like function and exposed continuously to asbestos fibers.

Aberrant expression of FoxP3 in a human T cell line possessing regulatory T cell‑like function and exposed continuously to asbestos fibers. Oncol Rep. 2018 Jun 07;: Authors: Maeda M, Matsuzaki H, Yamamoto S, Lee S, Kumagai-Takei N, Yoshitome K, Min Y, Sada N, Nishimura Y, Otsuki T Abstract Prompted by the known carcinogenic activity of asbestos, our investigations revealed that asbestos causes a reduction in antitumor immunity. One mechanism involves the enhancement of regulatory T (Treg) cell function and volume assayed using MT‑2 original cells (Org), an HTLV‑1 immortalized human T cell line which possesses Treg‑like function. Continuous and relatively low‑dose exposure of MT‑2 to asbestos fibers yielded sublines resistant to asbestos‑induced apoptosis and enhanced Treg function via cell‑cell contact mechanisms and increased the production of soluble factors such as interleukin (IL)‑10 and transforming growth factor (TGF)‑β. Additionally, cell cycle progression was accelerated in these sublines. Subsequently, the status of the Treg‑specific transcription factor FoxP3 was examined. Unexpectedly, FoxP3 mRNA levels decreased in the sublines, although significant changes in protein expression were absent. Methylation analysis of CpG sites located in the promoter region of FoxP3 in original MT‑2 cells and sublines showed almost complete methylation in Org and slight hypomethylation in the sublines. Although tr...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research