Aberrant determination of phenotypic markers in chronic lymphocytic leukemia (CLL) lymphocytes after cryopreservation

In a diagnostic setting, quantifying the expression of membrane proteins by flow cytometry is generally performed using fresh blood samples, in which the conformation of the epitope is likely to be in its native form. However, in a research environment, cells such as peripheral blood mononuclear cells (PBMCs) are often cryopreserved in liquid nitrogen and analyzed as needed. Cryogenic preservation requires the addition of cryoprotective reagents, most commonly dimethyl sulfoxide (DMSO), to protect the cells from damage during the preservation process [1].
Source: Experimental Hematology - Category: Hematology Authors: Tags: Brief Communications Source Type: research