DOXC-class 2-oxoglutarate-dependent dioxygenase in safflower: Gene characterization, transcript abundance, and correlation with flavonoids

Publication date: October 2018 Source:Biochemical Systematics and Ecology, Volume 80 Author(s): Yanhua Tu, Dongqiao Li, Lijiao Fan, Xinlei Jia, Dandan Guo, Hailiang Xin, Meili Guo DOXC is one of the 2-oxoglutarate-dependent dioxygenases (2OGDs) classes, which is involved in the biosynthesis of colorful flavonoids and plant hormones. Safflower (Carthamus tinctorius L.) is a commonly used plant with flavonoids. Herein, we focused on analyzing the characterization, transcript abundance, and correlation of DOXC-class 2OGDs in safflower with flavonoids for the first time. TBLASTN analysis showed that they were implicated in the biosynthetic pathway of ethylene (DOXC1 and DOXC2), indole-3-acetic acid (DOXC3, DOXC4, and DOXC5), flavonoids (DOXC6, DOXC7, and DOXC8), and scopolamine (DOXC9 and DOXC10). The transcript abundance of DOXC1, DOXC2, DOXC6, DOXC7, and DOXC10 response to methyl jasmonate (MeJA) in two safflower lines (orange-yellow-flowered safflower line with major quinochalcones and minor flavonols, white-flowered safflower line with major flavonols and without quinochalcones) had converse transcript level accumulation. Coexpression analyses displayed that the transcript level of DOXC1, DOXC2 and DOXC8 was positively correlated with hydroxysafflor yellow A, a major quinochalcone in orange-yellow-flowered safflower line. DOXC3, DOXC4, DOXC5, DOXC7, and DOXC9 displayed a marked positive correlation with kaempferol-3-O-β-D-glucoside, a main flavonols in white-flowere...
Source: Biochemical Systematics and Ecology - Category: Biochemistry Source Type: research