Antibiotic resistance could be countered by anti-bacterial viruses

Research found some patients had lower E coli levels after being given cocktail of ‘phages’Viruses that invade bacteria but leave human cells alone could help scientists find ways around the growing problem of antibiotic resistance, researchers have said. A study has showed that a cocktail of bacteriophages, or “phages”, resulted in no side effects when given to individuals with gastrointestinal problems and did not appear to greatly disrupt the diversity of microbes in the gut – the so-calledgut microbiota. But they did reduce levels of one marker of inflammation and certain problematic species of bacteria.Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Antibiotics Medical research Science Society Biology UK news Health Source Type: news

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Publication date: Available online 21 September 2018Source: PlasmidAuthor(s): Xiu-Zhong Zhang, Chang-Wei Lei, Jin-Xin Zeng, Yan-Peng Chen, Zhuang-Zhuang Kang, Yu-Long Wang, Xiao-Lan Ye, Xi-Wen Zhai, Hong-Ning WangAbstractWe have identified an IncX1 plasmid named pQJDSal1 from Salmonella enterica subsp. enterica serovar Pullorum (S. Pullorum). The plasmid is 67,685 bp in size and has 72 putative genes. pQJDSal1 harbors a conserved IncX1-type backbone with predicted regions for conjugation, replication and partitioning, as well as a toxin/antitoxin plasmid addiction system. Two regions (A and B) that have not been previous...
Source: Plasmid - Category: Biotechnology Source Type: research
In conclusion, the response of the cells to exposure elevated temperatures prior to inoculation into kefir affected the kinetics of the survival curves.
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: December 2018Source: LWT, Volume 98Author(s): Min-Jin Jeon, Jae-Won HaAbstractIn many UV sterilization studies, buffer solutions are used for investigating the inactivation mechanisms and evaluating the antimicrobial effect of UV light. The aim of this study was to investigate the effects of the type of buffer solution [phosphate-buffered saline (PBS) or peptone water (PW)] on UV inactivation of major foodborne pathogens, including Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes. A mixed culture cocktail was inoculated in PBS or PW and treated with UV-A, -B, o...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 22 September 2018Source: Journal of Molecular StructureAuthor(s): K. Saiadali Fathima, M. Sathiyendran, K. AnithaAbstractAn organic salt, 2-aminobenzimidazolium 5-nitro-2-hydroxybenzoate was synthesized and crystals were successfully grown from methanol using slow evaporation solution growth technique and recrystallization was done further to increase the purity of the crystal. The density of the grown crystals was evaluated by flotation method using carbon tetrachloride and xylene as solvents. Single crystal X-ray diffraction showed that the crystal is monoclinic system with P21/n space ...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
In this study, both strategies showed positive influence on the extracellular secretion of pullulanase. After fusing a negatively charged peptide at the N-terminal, the extracellular enzymatic activity increased by more than 4 times compared to the pullulanase without peptide fusion. By modifying the permeability of the cell wall, the extracellular enzymatic activity increased by 12 times. In addition, the two strategies were also used to improve the secretion efficiency of the truncated pullulanase ΔN106, which has higher enzymatic activity than the full-length protein. The strategies employed have valuable implicat...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Publication date: Available online 21 September 2018Source: Protein Expression and PurificationAuthor(s): Xiaojie Pei, Yaoguang Chang, Jingjing ShenAbstractAlginate is the major constituent of brown algae and a commercially important polysaccharide with wide applications. Alginate lyases are desired tools for degrading alginate. Based on the genome mining of marine bacterium Wenyingzhuangia funcanilytica, an alginate lyase Aly7B_Wf was discovered, cloned and expressed in Escherichia coli. Aly7B_Wf belonged to subfamily 6 of PL7 family. Its biochemical properties, kinetic constants, substrate specificity and degradation pat...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
In this study, we expressed and purified full-length NS2B from ZIKV in E. coli. Purified NS2B was then reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. It was found that compared to wild type NS2B, NS2B C11S mutation in LMPG exhibited dispersed cross peaks in the 1H-15N-HSQC spectrum, thereby suggesting the feasibility for structural characterization using solution NMR spectroscopy.
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
International travel is rising quickly worldwide. Many people travel to tropical and subtropical areas, where there has been increasing exposure of travelers to infectious pathogens. Ocular parasitic infections are more prevalent in these geographical areas and they can lead to morbidity and mortality, often due to late or misdiagnosis due to the unfamiliarity of health staff with thesediseases. This is an up-to-date comprehensive review article that familiarizes physicians with ocular signs and symptoms, treatment, prevention, and geographic distribution of some parasites associated with travel.Keywords:Acanthamoeba, Amer...
Source: International Journal of Preventive Medicine - Category: International Medicine & Public Health Source Type: research
Authors: Jenkins C Abstract Enteroaggregative Escherichia coli (EAEC, formerly known as "EAggEC") cause acute or persistent watery diarrhoea (with or without mucus) in children, predominantly in low-income countries, and are associated with travellers' diarrhoea in children and adults in middle and high income countries. The diverse nature of EAEC is such that not all strains cause disease. Conversely, certain strains of EAEC possess additional virulence determinants associated with the ability to cause severe diarrhoea and other symptoms, which might be life-threatening in vulnerable patients. The EAEC v...
Source: Current Topics in Microbiology and Immunology - Category: Microbiology Tags: Curr Top Microbiol Immunol Source Type: research
Abstract A method for the synthesis of β-lactam antibiotic cefazolin (CEZ) by enzymatic acylation of 7-amino-3-(5-methyl-l,3,4-thiadiazol-2-yl)thiomethyl-3-cephem-4-carboxylic acid (TDA) using immobilized cephalosporin-acid synthetase (IECASA) from recombinant E. coli strain VKPM B-12316 has been developed. A stepwise pH gradient designed on the basis of investigations on the solubility of components was applied for synthesis. This helped in avoiding the precipitation of TDA in the reaction when its initial concentration was high (150-200 mM). Thus, under optimal conditions a high yield of CEZ (relative ...
Source: Bioprocess and Biosystems Engineering - Category: Biomedical Engineering Authors: Tags: Bioprocess Biosyst Eng Source Type: research
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