Multi-parameter approach to evaluate the timing of memory status after 17DD-YF primary vaccination

by Christiane Costa-Pereira, Ana Carolina Campi-Azevedo, Jordana Grazziela Coelho-dos-Reis, Vanessa Peruhype-Magalh ães, Márcio Sobreira Silva Araújo, Lis Ribeiro do Vale Antonelli, Cristina Toscano Fonseca, Jandira Aparecida Lemos, Luiz Cosme Cote Malaquias, Matheus de Souza Gomes, Laurence Rodrigues Amaral, Maria Rios, Caren Chancey, Harold Richard Persi, Jorge Marcelo Pereira, Maria de Lourdes de Sousa Maia , Marcos da Silva Freire, Reinaldo de Menezes Martins, Akira Homma, Marisol Simões, Anna Yoshida Yamamura, Roberto Henrique Guedes Farias, Alessandro Pecego Martins Romano, Carla Magda Domingues, Pedro Luiz Tauil, Pedro Fernando Costa Vasconcelos, Iramaya Rodrigues Caldas, Luiz Antônio Camacho, An drea Teixeira-Carvalho, Olindo Assis Martins-Filho In this investigation, machine-enhanced techniques were applied to bring about scientific insights to identify a minimum set of phenotypic/functional memory-related biomarkers for post-vaccination follow-up upon yellow fever (YF) vaccination. For this purpose, memory status of circulating T-cells (Naïve/early-effector/Central-Memory/Effector-Memory) and B-cells (Naïve/non-Classical-Memory/Classical-Memory) along with the cytokine profile (IFN/TNF/IL-5/IL-10) were monitored before-NV(day0) and at distinct time-points after 17DD-YF primary vaccination—PV(day30-45); PV(year1-9) and PV(year1 0-11). A set of biomarkers (eEfCD4; EMCD4; CMCD19; EMCD8; IFNCD4; IL-5CD8; TNFCD4; IFNCD8; TNFCD8; IL-5CD19; IL-5CD4) were observed...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research