Genetic and Biochemical characterization of OXA-519, a novel OXA-48-like β-lactamase.

Genetic and Biochemical characterization of OXA-519, a novel OXA-48-like β-lactamase.
Antimicrob Agents Chemother. 2018 Jun 04;:
Authors: Dabos L, Bogaerts P, Bonnin RA, Zavala A, Sacré P, Iorga BI, Huang DT, Glupczynski Y, Naas T
Abstract
A multidrug-resistant K. pneumoniae 1210 isolate with reduced carbapenem susceptibility revealed the presence of a novel plasmid-encoded blaOXA-48-like gene, named blaOXA-519. The 60.7-kb plasmid (pOXA-519) was similar to the IncL-OXA-48 prototypical plasmid except for a ca. 2-kb deletion due to an IS1R insertion. OXA-519 differed from OXA-48 by a Val120Leu substitution, which resulted in an overall reduced ß-lactam-hydrolysis profile, except for ertapenem and meropenem that was increased. Thus, detection of OXA-519-producers using biochemical tests monitoring imipenem-hydrolysis will be difficult.
PMID: 29866857 [PubMed - as supplied by publisher]

Source: Antimicrobial Agents and Chemotherapy - June 4, 2018 Category: Microbiology Authors: Dabos L, Bogaerts P, Bonnin RA, Zavala A, Sacré P, Iorga BI, Huang DT, Glupczynski Y, Naas T Tags: Antimicrob Agents Chemother Source Type: research