Newfound effect of N-acetylaspartate in preventing and reversing aggregation of amyloid-beta in vitro.

Newfound effect of N-acetylaspartate in preventing and reversing aggregation of amyloid-beta in vitro.
Neurobiol Dis. 2018 May 31;:
Authors: Dollé JP, Rodgers JM, Browne KD, Troxler T, Gai F, Smith DH
Abstract
Although N-acetylaspartate (NAA) has long been recognized as the most abundant amino acid in neurons by far, its primary role has remained a mystery. Based on its unique tertiary structure, we explored the potential of NAA to modulate aggregation of amyloid-beta (Aβ) peptide 1-42 via multiple corroborating aggregation assays along with electron microscopy. Thioflavin-T fluorescence assay demonstrated that at physiological concentrations, NAA substantially inhibited the initiation of Aβ fibril formation. In addition, NAA added after 25 min of Aβ aggregation was shown to break up preformed fibrils. Electron microscopy analysis confirmed the absence of mature fibrils following NAA treatment. Furthermore, fluorescence correlation spectroscopy and dynamic light scattering measurements confirmed significant reductions in Aβ fibril hydrodynamic radius following treatment with NAA. These results suggest that physiological levels of NAA could play an important role in controlling Aβ aggregation in vivo where they are both found in the same neuronal compartments.
PMID: 29859874 [PubMed - as supplied by publisher]

Source: Neurobiology of Disease - May 31, 2018 Category: Neurology Authors: Dollé JP, Rodgers JM, Browne KD, Troxler T, Gai F, Smith DH Tags: Neurobiol Dis Source Type: research