Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in β-carboline skeleton biosynthesis

β-Carboline alkaloids (βCs), with tricyclic pyrido[3,4-b]indole rings, have important pharmacological and therapeutic value. In the biosynthesis of βCs, the Pictet–Spengler (PS) cyclization reaction is responsible for the formation of ring structures. McbB is one of a few enzymes that are known to catalyse PS cyclization. It can also catalyse decarboxylation and oxidation. Here, the expression, crystallization and preliminary data analysis of McbB are reported. The crystals diffracted to 2.10 Å resolution and belonged to the monoclinic space group P21, with unit-cell parameters a = 66.06, b = 85.48, c = 106.19 Å, α = 90.00, β = 106.77, γ = 90.00°. These results provide a basis for solving the crystal structure and elucidating the catalytic mechanism for McbB.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: β -carboline alkaloids Pictet – Spengler cyclization McbB Marinactinospora thermotolerans crystallization communications Source Type: research

Related Links:

Publication date: Available online 27 March 2020Source: Sensing and Bio-Sensing ResearchAuthor(s): Md. Jayed Bin Murshed Leon, Md. Ahasan Kabir
Source: Sensing and Bio Sensing Research - Category: Research Source Type: research
Authors: Mendez J PMID: 32208918 [PubMed - as supplied by publisher]
Source: Gerontology and Geriatrics Education - Category: Geriatrics Tags: Gerontol Geriatr Educ Source Type: research
Publication date: Available online 27 March 2020Source: Neuroimaging Clinics of North AmericaAuthor(s): Heather L. Green, J. Christopher Edgar, Junko Matsuzaki, Timothy P.L. Roberts
Source: Neuroimaging Clinics of North America - Category: Radiology Source Type: research
Publication date: Available online 27 March 2020Source: NeuroImage: ClinicalAuthor(s): Sarah C. Hellewell, Vy Phuong Brenda Nguyen, Ruchira Jayasena, Thomas Welton, Stuart M. Grieve
Source: NeuroImage: Clinical - Category: Radiology Source Type: research
Publication date: Available online 28 March 2020Source: Journal of Vascular and Interventional RadiologyAuthor(s): Jeffrey C. Cruz, Daniel B. Brown
Source: Journal of Vascular and Interventional Radiology - Category: Radiology Source Type: research
Publication date: Available online 28 March 2020Source: Journal of Vascular and Interventional RadiologyAuthor(s): Karen T. Brown
Source: Journal of Vascular and Interventional Radiology - Category: Radiology Source Type: research
Publication date: Available online 27 March 2020Source: Journal of Vascular and Interventional RadiologyAuthor(s): Jin Woo Choi, Jeong Min Lee, Hyo-Cheol Kim, Myungsu Lee, Saebeom Hur, Hwan Jun Jae, Jin Wook Chung
Source: Journal of Vascular and Interventional Radiology - Category: Radiology Source Type: research
Publication date: Available online 28 March 2020Source: Academic RadiologyAuthor(s): Stanzione Arnaldo, Renato Cuocolo, Del Grosso Renata, Nardiello Anna, Romeo Valeria, Travaglino Antonio, Raffone Antonio, Bifulco Giuseppe, Zullo Fulvio, Insabato Luigi, Maurea Simone, Pier Paolo Mainenti
Source: Academic Radiology - Category: Radiology Source Type: research
33 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Respir Crit Care Med These pubmed results were generated on 2020/03/28PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Source: Am J Respir Crit Car... - Category: Intensive Care Tags: Report Source Type: research
Publication date: Available online 27 March 2020Source: Social Science &MedicineAuthor(s): Emma Elizabeth Phelps, Elizabeth Tutton, Xavier Griffin, Janis Baird
Source: Social Science and Medicine - Category: Psychiatry & Psychology Source Type: research
More News: Biochemistry