Influence of early zoledronic acid administration on bone marrow fat in ovariectomized rats.

Influence of early zoledronic acid administration on bone marrow fat in ovariectomized rats. Endocrinology. 2014 Sep 22;:en20141359 Authors: Li GW, Xu Z, Chang SX, Zhou L, Wang XY, Nian H, Shi X Abstract Although the primary target cell of bisphosphonates is the osteoclast, increasing attention is being given to other effector cells influenced by bisphosphonates, such as osteoblasts and marrow adipocytes. Early zoledronic acid (ZA) treatment to ovariectomized (OVX) rats has been found to fully preserve bone microarchitecture over time. However, little is known regarding the influence of ZA on marrow adipogenesis. The purpose of this study was to monitor the ability of early administration of ZA in restoring marrow adiposity in an estrogen-deficient rat model. Thirty female Sprague-Dawley rats were randomly divided into SHAM-operated, OVX + vehicle, and OVX + ZA groups (n=10/group). Dual-energy X-ray absorptiometry and water/fat MRI were performed at baseline, 6 weeks and 12 weeks after treatment to assess bone mineral density (BMD) and marrow fat fraction. Serum biochemical markers, bone remodeling and marrow adipocyte parameters were analyzed using biochemistry, histomorphometry and histopathology, respectively. The expression levels of osteoblast, adipocyte and osteoclast-related genes in bone marrow were assessed using RT-PCR. The OVX rats showed marked bone loss, first detected at 12 weeks, but estrogen deficiency resulted in a r...
Source: Endocrinology - Category: Endocrinology Authors: Tags: Endocrinology Source Type: research