Genome characterization of Sugarcane Yellow Leaf Virus with special reference to RNAi based molecular breeding
Publication date: July 2018 Source:Microbial Pathogenesis, Volume 120 Author(s): Farghama Khalil, Xu Yueyu, Xiao Naiyan, Liu Di, Muhammad Tayyab, Wang Hengbo, Waqar Islam, Saeed Rauf, Chen Pinghua Sugarcane is an essential crop for sugar and biofuel. Globally, its production is severely affected by sugarcane yellow leaf disease (SCYLD) caused by Sugarcane Yellow Leaf Virus (SCYLV). Many aphid vectors are involved in the spread of the disease which reduced the effectiveness of cultural and chemical management. Empirical methods of plant breeding such as introgression from wild and cultivated germplasm were not possible or at least challenging due to the absence of resistance in cultivated and wild germplasm of sugarcane. RNA interference (RNAi) transformation is an effective method to create virus-resistant varieties. Nevertheless, limited progress has been made due to lack of comprehensive research program on SCYLV based on RNAi technique. In order to show improvement and to propose future strategies for the feasibility of the RNAi technique to cope SCYLV, genome-wide consensus sequences of SCYLV were analyzed through GenBank. The coverage rates of every consensus sequence in SCYLV isolates were calculated to evaluate their practicability. Our analysis showed that single consensus sequence from SCYLV could not work well for RNAi based sugarcane breeding programs. This may be due to high mutation rate and continuous recombination within and between various viral str...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research