Polymorphisms of genes related to metabolism of lead (Pb) are associated with the metal body burden and with biomarkers of oxidative stress

Publication date: Available online 25 May 2018 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis Author(s): Willian Robert Gomes, Paula Pícoli Devóz, Bruno Limonti Cano Luiz, Denise Grotto, Bruno Lemos Batista, Fernando Barbosa, Gustavo Rafael Mazzaron Barcelos Individual susceptibility to the toxic effects induced by exposure to lead (Pb) may be affected by several variables, such as environmental factors, as well as intrinsic variations among the individuals, which are hypothetically associated to genetic differences in enzymes metabolizing the metal. Aim of the present study was to evaluate the effects of polymorphisms of glutathione (GSH)-genes related to the antioxidant status and Pb metabolism (GCLC, rs17883901 and GCLM, rs41303970) on Pb levels in blood (B-Pb) and plasma (P-Pb), as well as Pb-related effects on activity of glutathione-peroxidase (GPX) and on GSH concentrations. A cross-sectional study with 236 adults (men, >18 years old) was carried out with workers from automotive battery factories, Brazil. B-Pb and P-Pb were determined by ICP-MS; blood GPX and GSH were determined by spectrophotometry and qPCR TaqMan assays were used for genotyping. A questionnaire was applied in order to collect socio-demographic, lifestyle and time of exposure. The mean B-Pb level was 211 ± 118 μg/L and P-Pb was 6.05 ± 7.13 μg/L. GCLM are associated with changes of B-Pb and P-Pb; individuals who carry at least one polymor...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research