Review article A translational view of cells' secretome analysis - from untargeted proteomic to potential circulating biomarkers.

Review article A translational view of cells' secretome analysis - from untargeted proteomic to potential circulating biomarkers.
Biochimie. 2018 May 18;:
Authors: Anjo SI, Manadas B
Abstract
The identification of circulating biomarkers capable of being used as routine for diagnosis, prognosis, risk stratification and therapeutic monitoring is still the major aim at clinical proteomics. However, the direct search in blood samples or other clinical relevant biofluids is frequently associated with technical limitations that makes the biomarker discovery process extremely difficult when using these types of samples. In this sense, the use of different approaches, such as the analysis of cells' secretomes, can be an important and promising complementary method for the identification and pre-selection of potential circulating biomarkers. Therefore, the present review is focused on the previously published works exclusively devoted to the use of cells' secretomes for the identification of new circulating biomarkers. Considering these publications, the translational workflow used to identify potential protein circulating biomarkers from untargeted cells' secretome screenings is presented and discussed in this review. This translational workflow follows a 3-phase pipeline (discovery, verification and validation phase) similar to the "triangular strategy" already applied in biomarker discovery using blood or other clinical samples. For each p...

Source: Biochimie - May 18, 2018 Category: Biochemistry Authors: Anjo SI, Manadas B Tags: Biochimie Source Type: research