MicroRNA ‑20b‑5p promotes ventricular remodeling by targeting the TGF‑β/Smad signaling pathway in a rat model of ischemia‑reperfusion injury.

MicroRNA‑20b‑5p promotes ventricular remodeling by targeting the TGF‑β/Smad signaling pathway in a rat model of ischemia‑reperfusion injury. Int J Mol Med. 2018 May 18;: Authors: Liang ZG, Yao H, Xie RS, Gong CL, Tian Y Abstract Myocardial ischemic injury results from severe impairment of the coronary blood supply and may lead to metabolic and ultrastructural changes, thereby causing irreversible damage. MicroRNA (miR)‑20b‑5p has been demonstrated to be involved in malignancies of the breast, colorectum, stomach, blood and oropharynx. The present study aimed to investigate the effects of miR‑20b‑5p on ventricular remodeling following myocardial ischemia‑reperfusion (IR) injury in rats by targeting small mothers against decapentaplegic homolog 7 (Smad7) via the transforming growth factor‑β (TGF‑β)/Smad signaling pathway. A total of 70 adult male Sprague‑Dawley rats were divided into seven groups: Sham group, IR group, negative control group, miR‑20b‑5p mimics group, miR‑20b‑5p inhibitors group, small interfering RNA (siRNA)‑Smad7 group, and miR‑20b‑5p inhibitors + siRNA‑Smad7 group. Dual luciferase reporter gene assays were used to verify the association between miR‑20b‑5p and Smad7. Myocardial infarction size, myocardial collagen volume fraction and perivascular collagen area were detected separately using triphenyltetrazolium chloride and Masson's staining. The rate of positive express...
Source: International Journal of Molecular Medicine - Category: Molecular Biology Authors: Tags: Int J Mol Med Source Type: research