Development of dried serum spot sampling techniques for the assessment of trace elements in serum samples by LA-ICP-MS.

Development of dried serum spot sampling techniques for the assessment of trace elements in serum samples by LA-ICP-MS. Talanta. 2018 Aug 15;186:169-175 Authors: Chantada-Vázquez MP, Moreda-Piñeiro J, Cantarero-Roldán A, Bermejo-Barrera P, Moreda-Piñeiro A Abstract A novel approach for serum analysis by dried matrix spot (DMS) technique is proposed. The methodology consists of sampling filter paper discs (2.7 mm in diameter) containing the large amount of serum retained after a single spotting. Several oxidizers (sodium chlorate, sodium azide, acetic acid, formic acid, 1-butyl-3-methylimidazoliumm chloride/bromide) were tested (oxidizers premixed with the sample before spotting, and papers previously soaked in concentrated additive/oxidizer solutions). Direct multi-element determination (Al, Be, Ca, Cu, Fe, K, Li, Mg, Mn, Mo, Na, P, Rb, Se, V, and Zn) in dried serum spots at very low levels was therefore assessed by laser ablation (LA) coupled with inductively coupled plasma - mass spectrometry (ICP-MS). Laser ablation was performed using a focused Nd: YAG laser beam in lineal scan mode (wavelength 213 nm, laser fluency 2.2 J cm-2, repetition rate 20 Hz, laser spot diameter 90 µm, depth 0 µm, scanning speed 12 µm s-1). Matrix-matched calibration mode and 13C as internal standard (for signal intensities normalization) was used throughout the work. Limits of quantification were found to be from 21 µg L-1 t...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research