Graphene oxide-based composite monolith as new sorbent for the on-line solid phase extraction and high performance liquid chromatography determination of ß-sitosterol in food samples.

Graphene oxide-based composite monolith as new sorbent for the on-line solid phase extraction and high performance liquid chromatography determination of ß-sitosterol in food samples.
Talanta. 2018 Aug 15;186:200-205
Authors: Cui B, Guo B, Wang H, Zhang D, Liu H, Bai L, Yan H, Han D
Abstract
A composite monolithic column was prepared by redox initiation method for the on-line purification and enrichment of β-sitosterol, in which graphene oxide (GO) was embedded. The obtained monolithic column was characterized by scanning electron microscopy (SEM) and nitrogen adsorption-desorption isotherm measurement, which indicated that the monolith possessed characteristics of porous structure and high permeability. Under the optimum conditions for extraction and determination, the calibration equation was y = 47.92 × -0.1391; the linear range was 0.008-1.0 mg mL-1; the linear regression coefficient was 0.998; the limit of detection (LOD) is 2.4 μg mL-1; the limit of quantitation (LOQ) was 8 μg mL-1; precisions for intra-day and inter-day assays presented as relative standard deviations were less than 4.3% and 6.8%, respectively. Under the selective conditions, the enrichment factor of the method was 119. The recovery was in the range of 80.40-98.00%. Moreover, the adsorption amount of the monolith was compared with silica gel-C18 adsorbent and the monolith without graphene oxide being embedded. The polymerization monoli...

Source: Talanta - May 24, 2018 Category: Chemistry Authors: Cui B, Guo B, Wang H, Zhang D, Liu H, Bai L, Yan H, Han D Tags: Talanta Source Type: research