Highly selective and sensitive chemiluminescence biosensor for adenosine detection based on carbon quantum dots catalyzing luminescence released from aptamers functionalized graphene@magnetic β-cyclodextrin polymers.

Highly selective and sensitive chemiluminescence biosensor for adenosine detection based on carbon quantum dots catalyzing luminescence released from aptamers functionalized graphene@magnetic β-cyclodextrin polymers. Talanta. 2018 Aug 15;186:238-247 Authors: Sun Y, Ding C, Lin Y, Sun W, Liu H, Zhu X, Dai Y, Luo C Abstract In this work, a highly selective and sensitive chemiluminescence (CL) biosensor was prepared for adenosine (AD) detection based on carbon quantum dots (CQDs) catalyzing the CL system of luminol-H2O2 under alkaline environment and CQDs was released from the surface of AD aptamers functionalized graphene @ magnetic β-cyclodextrin polymers (GO@Fe3O4@β-CD@A-Apt). Firstly, GO@Fe3O4@β-CD and CQDs were prepared and characterized by transmission electron microscopy (TEM), scanning electron microscope (SEM), UV-Vis absorption spectra (UV), fluorescence spectra (FL), fourier transform infrared (FTIR) and X-ray powder diffraction (XRD). For GO@Fe3O4@β-CD, Fe3O4 was easy to separate, GO had good biocompatibility and large specific surface area, and β-CD further increased the specific surface area of the adenosine polymers (A-Apt) to provided larger binding sites to A-Apt. Then, A-Apt was modified on the surface of GO@Fe3O4@β-CD while CQDs was modified by ssDNA (a single stranded DNA partially complementary to A-Apt). The immobilization property (GO@Fe3O4@β-CD to A-Apt) and the adsorption property (GO@Fe3O4@β-CD@A-Apt t...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research